An image-based, dual fluorescence reporter assay to evaluate the efficacy of shRNA for gene silencing at the single-cell level
RNA interference (RNAi) is widely used to suppress gene expression in a specific manner. The efficacy of RNAi is mainly dependent on the sequence of small interfering RNA (siRNA) in relation to the target mRNA. Although several algorithms have been developed for the design of siRNA, it is still diff...
| Main Authors: | , |
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| Format: | Online |
| Language: | English |
| Published: |
F1000Research
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983939/ |