Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens

Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens

RNAi screening using pooled shRNA libraries is a valuable tool for identifying genetic regulators of biological processes. However, for a successful pooled shRNA screen, it is imperative to thoroughly optimize experimental conditions to obtain reproducible data. Here we performed viability screens w...

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Bibliographic Details
Main Authors: Strezoska, Žaklina, Licon, Abel, Haimes, Josh, Spayd, Katie Jansen, Patel, Kruti M., Sullivan, Kevin, Jastrzebski, Katarzyna, Simpson, Kaylene J., Leake, Devin, van Brabant Smith, Anja, Vermeulen, Annaleen
Format: Online
Language:English
Published: Public Library of Science 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411659/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3411659/

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