The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice
The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. The positional cloning of the mutation revealed a deletion in a gene encoding a protein disulphide isomerase-like enzyme (PDIL1-1). In the wild type, PDIL1-1 was expressed...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2012
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245461/ |
| id |
pubmed-3245461 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-32454612011-12-23 The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice Han, Xiaohua Wang, Yihua Liu, Xi Jiang, Ling Ren, Yulong Liu, Feng Peng, Cheng Li, Jingjing Jin, Ximing Wu, Fuqing Wang, Jiulin Guo, Xiuping Zhang, Xin Cheng, Zhijun Wan, Jianmin Research Papers The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. The positional cloning of the mutation revealed a deletion in a gene encoding a protein disulphide isomerase-like enzyme (PDIL1-1). In the wild type, PDIL1-1 was expressed throughout the plant, but most intensely in the developing grain. In T3612, its expression was abolished, resulting in a decrease in the activity of plastidial phosphorylase and pullulanase, and an increase in that of soluble starch synthase I and ADP-glucose pyrophosphorylase. The amylopectin in the T3612 endosperm showed an increase in chains with a degree of polymerization 8–13 compared with the wild type. The expression in the mutant's endosperm of certain endoplasmic reticulum stress-responsive genes was noticeably elevated. PDIL1-1 appears to play an important role in starch synthesis. Its absence is associated with endoplasmic reticulum stress in the endosperm, which is likely to underlie the formation of the floury endosperm in the T3612 mutant. Oxford University Press 2012-01 2011-10-06 /pmc/articles/PMC3245461/ /pubmed/21984651 http://dx.doi.org/10.1093/jxb/err262 Text en © 2011 The Author(s). http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Han, Xiaohua Wang, Yihua Liu, Xi Jiang, Ling Ren, Yulong Liu, Feng Peng, Cheng Li, Jingjing Jin, Ximing Wu, Fuqing Wang, Jiulin Guo, Xiuping Zhang, Xin Cheng, Zhijun Wan, Jianmin |
| spellingShingle |
Han, Xiaohua Wang, Yihua Liu, Xi Jiang, Ling Ren, Yulong Liu, Feng Peng, Cheng Li, Jingjing Jin, Ximing Wu, Fuqing Wang, Jiulin Guo, Xiuping Zhang, Xin Cheng, Zhijun Wan, Jianmin The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| author_facet |
Han, Xiaohua Wang, Yihua Liu, Xi Jiang, Ling Ren, Yulong Liu, Feng Peng, Cheng Li, Jingjing Jin, Ximing Wu, Fuqing Wang, Jiulin Guo, Xiuping Zhang, Xin Cheng, Zhijun Wan, Jianmin |
| author_sort |
Han, Xiaohua |
| title |
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| title_short |
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| title_full |
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| title_fullStr |
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| title_full_unstemmed |
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| title_sort |
failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice |
| description |
The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. The positional cloning of the mutation revealed a deletion in a gene encoding a protein disulphide isomerase-like enzyme (PDIL1-1). In the wild type, PDIL1-1 was expressed throughout the plant, but most intensely in the developing grain. In T3612, its expression was abolished, resulting in a decrease in the activity of plastidial phosphorylase and pullulanase, and an increase in that of soluble starch synthase I and ADP-glucose pyrophosphorylase. The amylopectin in the T3612 endosperm showed an increase in chains with a degree of polymerization 8–13 compared with the wild type. The expression in the mutant's endosperm of certain endoplasmic reticulum stress-responsive genes was noticeably elevated. PDIL1-1 appears to play an important role in starch synthesis. Its absence is associated with endoplasmic reticulum stress in the endosperm, which is likely to underlie the formation of the floury endosperm in the T3612 mutant. |
| publisher |
Oxford University Press |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245461/ |
| _version_ |
1611496405768077312 |