Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells
Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integratin...
Main Authors: | Wilber, Andrew, Ulloa Montoya, Fernando, Hammer, Luke, Moriarity, Branden S., Geurts, Aron M., Largaespada, David A., Verfaillie, Catherine M., McIvor, R. Scott, Lakshmipathy, Uma |
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Format: | Online |
Language: | English |
Published: |
SAGE-Hindawi Access to Research
2011
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184415/ |
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