Efficient Non-Viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells

Non-viral integrating systems, PhiC31 phage integrase (ϕC31), and Sleeping Beauty transposase (SB), provide an effective method for ex vivo gene delivery into cells. Here, we used a plasmid-encoding GFP and neomycin phosphotransferase along with recognition sequences for both ϕC31 and SB integratin...

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Bibliographic Details
Main Authors: Wilber, Andrew, Ulloa Montoya, Fernando, Hammer, Luke, Moriarity, Branden S., Geurts, Aron M., Largaespada, David A., Verfaillie, Catherine M., McIvor, R. Scott, Lakshmipathy, Uma
Format: Online
Language:English
Published: SAGE-Hindawi Access to Research 2011
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184415/