Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA

by Gutjahr, Alice, Xu, Shuang-yong
Published 2014

Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease

by Xu, Shuang-yong, Gupta, Yogesh K.
Published 2013

Natural and engineered nicking endonucleases—from cleavage mechanism to engineering of strand-specificity

by Chan, Siu-Hong, Stoddard, Barry L., Xu, Shuang-yong
Published 2011

The Role of the Methyltransferase Domain of Bifunctional Restriction Enzyme RM.BpuSI in Cleavage Activity

by Sarrade-Loucheur, Arthur, Xu, Shuang-yong, Chan, Siu-Hong
Published 2013

A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300

by Xu, Shuang-yong, Corvaglia, Anna R., Chan, Siu-Hong, Zheng, Yu, Linder, Patrick
Published 2011

Efficient isolation of targeted Caenorhabditis elegans deletion strains using highly thermostable restriction endonucleases and PCR

by Wei, Aguan, Yuan, Alex, Fawcett, Gloria, Butler, Alice, Davis, Theodore, Xu, Shuang-yong, Salkoff, Lawrence
Published 2005

Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities

by Chan, Siu-hong, Bao, Yongming, Ciszak, Ewa, Laget, Sophie, Xu, Shuang-yong
Published 2007

Characterization of cleavage intermediate and star sites of RM.Tth111II

by Zhu, Zhenyu, Guan, Shengxi, Robinson, Derek, Fezzazi, Hanna El, Quimby, Aine, Xu, Shuang-yong
Published 2014

Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I

by Gupta, Yogesh K., Chan, Siu-Hong, Xu, Shuang-yong, Aggarwal, Aneel K.
Published 2015

Expression and purification of the modification-dependent restriction enzyme BisI and its homologous enzymes

by Xu, Shuang-yong, Klein, Pernelle, Degtyarev, Sergey Kh., Roberts, Richard J.
Published 2016

Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants

by Samuelson, James C., Morgan, Richard D., Benner, Jack S., Claus, Toby E., Packard, Stephanie L., Xu, Shuang-yong
Published 2006

Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI

by Xu, Shuang-Yong, Zhu, Zhenyu, Zhang, Penghua, Chan, Siu-Hong, Samuelson, James C., Xiao, Jianping, Ingalls, Debra, Wilson, Geoffrey G.
Published 2007

Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI

by Shen, Betty W., Xu, Derrick, Chan, Siu-Hong, Zheng, Yu, Zhu, Zhenyu, Xu, Shuang-yong, Stoddard, Barry L.
Published 2011

Increasing cleavage specificity and activity of restriction endonuclease KpnI

by Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
Published 2013

Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference

by He, Xinyi, Hull, Victoria, Thomas, Julie A., Fu, Xiaoqing, Gidwani, Sonal, Gupta, Yogesh K., Black, Lindsay W., Xu, Shuang-yong
Published 2015

Structure Determination and Biochemical Characterization of a Putative HNH Endonuclease from Geobacter metallireducens GS-15

by Xu, Shuang-yong, Kuzin, Alexandre P., Seetharaman, Jayaraman, Gutjahr, Alice, Chan, Siu-Hong, Chen, Yang, Xiao, Rong, Acton, Thomas B., Montelione, Gaetano T., Tong, Liang
Published 2013

Complete Genome Sequence Analysis of Bacillus subtilis T30

by Xu, Shuang-yong, Boitano, Matthew, Clark, Tyson A., Vincze, Tamas, Fomenkov, Alexey, Kumar, Sanjay, Too, Priscilla Hiu-Mei, Gonchar, Danila, Degtyarev, Sergey K., Roberts, Richard J.
Published 2015

Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR

by Ghindilis, Andrey L., Smith, Maria W., Simon, Holly M., Seoudi, Ihab A., Yazvenko, Nina S., Murray, Iain A., Fu, Xiaoqing, Smith, Kenneth, Jen-Jacobson, Linda, Xu, Shuang-yong
Published 2015

Structure and mutagenesis of the DNA modification-dependent restriction endonuclease AspBHI

by Horton, John R., Nugent, Rebecca L., Li, Andrew, Mabuchi, Megumu Yamada, Fomenkov, Alexey, Cohen-Karni, Devora, Griggs, Rose M., Zhang, Xing, Wilson, Geoffrey G., Zheng, Yu, Xu, Shuang-yong, Cheng, Xiaodong
Published 2014

The COMBREX Project: Design, Methodology, and Initial Results

by Anton, Brian P., Chang, Yi-Chien, Brown, Peter, Choi, Han-Pil, Faller, Lina L., Guleria, Jyotsna, Hu, Zhenjun, Klitgord, Niels, Levy-Moonshine, Ami, Maksad, Almaz, Mazumdar, Varun, McGettrick, Mark, Osmani, Lais, Pokrzywa, Revonda, Rachlin, John, Swaminathan, Rajeswari, Allen, Benjamin, Housman, Genevieve, Monahan, Caitlin, Rochussen, Krista, Tao, Kevin, Bhagwat, Ashok S., Brenner, Steven E., Columbus, Linda, de Crécy-Lagard, Valérie, Ferguson, Donald, Fomenkov, Alexey, Gadda, Giovanni, Morgan, Richard D., Osterman, Andrei L., Rodionov, Dmitry A., Rodionova, Irina A., Rudd, Kenneth E., Söll, Dieter, Spain, James, Xu, Shuang-yong, Bateman, Alex, Blumenthal, Robert M., Bollinger, J. Martin, Chang, Woo-Suk, Ferrer, Manuel, Friedberg, Iddo, Galperin, Michael Y., Gobeill, Julien, Haft, Daniel, Hunt, John, Karp, Peter, Klimke, William, Krebs, Carsten, Macelis, Dana, Madupu, Ramana, Martin, Maria J., Miller, Jeffrey H., O'Donovan, Claire, Palsson, Bernhard, Ruch, Patrick, Setterdahl, Aaron, Sutton, Granger, Tate, John, Yakunin, Alexander, Tchigvintsev, Dmitri, Plata, Germán, Hu, Jie, Greiner, Russell, Horn, David, Sjölander, Kimmen, Salzberg, Steven L., Vitkup, Dennis, Letovsky, Stanley, Segrè, Daniel, DeLisi, Charles, Roberts, Richard J., Steffen, Martin, Kasif, Simon
Published 2013