A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300
A gene encoding a putative DNA helicase from Staphylococcus aureus USA300 was cloned and expressed in Escherichia coli. The protein was purified to over 90% purity by chromatography. The purified enzyme, SauUSI, predominantly cleaves modified DNA containing 5mC and 5-hydroxymethylcytosine. Cleavage...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Oxford University Press
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141236/ |