Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR

Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR

An alternative to qPCR was developed for nucleic acid assays, involving signal rather than target amplification. The new technology, Restriction Cascade Exponential Amplification (RCEA), relies on specific cleavage of probe-target hybrids by restriction endonucleases (REase). Two mutant REases for a...

Full description

Bibliographic Details
Main Authors: Ghindilis, Andrey L., Smith, Maria W., Simon, Holly M., Seoudi, Ihab A., Yazvenko, Nina S., Murray, Iain A., Fu, Xiaoqing, Smith, Kenneth, Jen-Jacobson, Linda, Xu, Shuang-yong
Format: Online
Language:English
Published: Nature Publishing Group 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291554/

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291554/

Similar Items