Resources for the design of CRISPR gene editing experiments

by Graham, Daniel B., Root, David E.
Published 2015

Identification of RPS14 as a 5q- syndrome gene by RNA interference screen

by Ebert, Benjamin L., Pretz, Jennifer, Bosco, Jocelyn, Chang, Cindy Y., Tamayo, Pablo, Galili, Naomi, Raza, Azra, Root, David E., Attar, Eyal, Ellis, Steven R., Golub, Todd R.
Published 2008

A Targeted RNA Interference Screen Reveals Novel Epigenetic Factors That Regulate Herpesviral Gene Expression

by Oh, Hyung Suk, Bryant, Kevin F., Nieland, Thomas J. F., Mazumder, Aprotim, Bagul, Mukta, Bathe, Mark, Root, David E., Knipe, David M.
Published 2014

High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons

by Nieland, Thomas J. F., Logan, David J., Saulnier, Jessica, Lam, Daniel, Johnson, Caroline, Root, David E., Carpenter, Anne E., Sabatini, Bernardo L.
Published 2014

Morphological Profiles of RNAi-Induced Gene Knockdown Are Highly Reproducible but Dominated by Seed Effects

by Singh, Shantanu, Wu, Xiaoyun, Ljosa, Vebjorn, Bray, Mark-Anthony, Piccioni, Federica, Root, David E., Doench, John G., Boehm, Jesse S., Carpenter, Anne E.
Published 2015

A melanocyte lineage program confers resistance to MAP kinase pathway inhibition

by Johannessen, Cory M., Johnson, Laura A., Piccioni, Federica, Townes, Aisha, Frederick, Dennie T., Donahue, Melanie K., Narayan, Rajiv, Flaherty, Keith T., Wargo, Jennifer A., Root, David E., Garraway, Levi A.
Published 2013

lincRNAs act in the circuitry controlling pluripotency and differentiation

by Guttman, Mitchell, Donaghey, Julie, Carey, Bryce W., Garber, Manuel, Grenier, Jennifer K., Munson, Glen, Young, Geneva, Lucas, Anne Bergstrom, Ach, Robert, Bruhn, Laurakay, Yang, Xiaoping, Amit, Ido, Meissner, Alexander, Regev, Aviv, Rinn, John L., Root, David E., Lander, Eric S.
Published 2011

The Master Regulator of the Cellular Stress Response (HSF1) Is Critical for Orthopoxvirus Infection

by Filone, Claire Marie, Caballero, Ignacio S., Dower, Ken, Mendillo, Marc L., Cowley, Glenn S., Santagata, Sandro, Rozelle, Daniel K., Yen, Judy, Rubins, Kathleen H., Hacohen, Nir, Root, David E., Hensley, Lisa E., Connor, John
Published 2014

TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity

by Ramirez-Ortiz, Zaida G., Prasad, Amit, Griffith, Jason W., Pendergraft, William F., Cowley, Glenn S., Root, David E., Tai, Melissa, Luster, Andrew D., Khoury, Joseph El, Hacohen, Nir, Means, Terry K.
Published 2015

Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst

by Graham, Daniel B., Becker, Christine E., Doan, Aivi, Goel, Gautam, Villablanca, Eduardo J., Knights, Dan, Mok, Amanda, Ng, Aylwin C.Y., Doench, John G., Root, David E., Clish, Clary B., Xavier, Ramnik J.
Published 2015

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

by Doench, John G., Fusi, Nicolo, Sullender, Meagan, Hegde, Mudra, Vaimberg, Emma W., Donovan, Katherine F., Smith, Ian, Tothova, Zuzana, Wilen, Craig, Orchard, Robert, Virgin, Herbert W., Listgarten, Jennifer, Root, David E.
Published 2016

A public genome-scale lentiviral expression library of human ORFs

by Yang, Xiaoping, Boehm, Jesse S, Yang, Xinping, Salehi-Ashtiani, Kourosh, Hao, Tong, Shen, Yun, Lubonja, Rakela, Thomas, Sapana R, Alkan, Ozan, Bhimdi, Tashfeen, Green, Thomas M, Johannessen, Cory M, Silver, Serena, Nguyen, Cindy, Murray, Ryan R., Hieronymus, Haley, Balcha, Dawit, Fan, Changyu, Lin, Chenwei, Ghamsari, Lila, Vidal, Marc, Hahn, William C, Hill, David E, Root, David E
Published 2011

A comprehensive platform for highly multiplexed mammalian functional genetic screens

by Ketela, Troy, Heisler, Lawrence E, Brown, Kevin R, Ammar, Ron, Kasimer, Dahlia, Surendra, Anuradha, Ericson, Elke, Blakely, Kim, Karamboulas, Dina, Smith, Andrew M, Durbic, Tanja, Arnoldo, Anthony, Cheung-Ong, Kahlin, Koh, Judice LY, Gopal, Shuba, Cowley, Glenn S, Yang, Xiaoping, Grenier, Jennifer K, Giaever, Guri, Root, David E, Moffat, Jason, Nislow, Corey
Published 2011

Csnk1a1 inhibition has p53-dependent therapeutic efficacy in acute myeloid leukemia

by Järås, Marcus, Miller, Peter G., Chu, Lisa P., Puram, Rishi V., Fink, Emma C., Schneider, Rebekka K., Al-Shahrour, Fatima, Peña, Pablo, Breyfogle, L. Jordan, Hartwell, Kimberly A., McConkey, Marie E., Cowley, Glenn S., Root, David E., Kharas, Michael G., Mullally, Ann, Ebert, Benjamin L.
Published 2014

Functional genomics reveals serine synthesis is essential in PHGDH-amplified breast cancer

by Possemato, Richard, Marks, Kevin M., Shaul, Yoav D., Pacold, Michael E., Kim, Dohoon, Birsoy, Kivanç, Sethumadhavan, Shalini, Woo, Hin-Koon, Jang, Hyun G., Jha, Abhishek K., Chen, Walter W., Barrett, Francesca G., Stransky, Nicolas, Tsun, Zhi-Yang, Cowley, Glenn S., Barretina, Jordi, Kalaany, Nada Y., Hsu, Peggy P., Ottina, Kathleen, Chan, Albert M., Yuan, Bingbing, Garraway, Levi A., Root, David E., Mino-Kenudson, Mari, Brachtel, Elena F., Driggers, Edward M., Sabatini, David M.
Published 2011

The Bromodomain Protein Brd4 Insulates Chromatin from DNA Damage Signaling

by Floyd, Scott R., Pacold, Michael E., Huang, Qiuying, Clarke, Scott M., Lam, Fred C., Cannell, Ian G., Bryson, Bryan D., Rameseder, Jonathan, Lee, Michael J., Blake, Emily J., Fydrych, Anna, Ho, Richard, Greenberger, Benjamin A., Chen, Grace C., Maffa, Amanda, Del Rosario, Amanda M., Root, David E., Carpenter, Anne E., Hahn, William C., Sabatini, David M., Chen, Clark C., White, Forest M., Bradner, James E., Yaffe, Michael B.
Published 2013

COT/MAP3K8 drives resistance to RAF inhibition through MAP kinase pathway reactivation

by Johannessen, Cory M., Boehm, Jesse S., Kim, So Young, Thomas, Sapana R., Wardwell, Leslie, Johnson, Laura A., Emery, Caroline M., Stransky, Nicolas, Cogdill, Alexandria P., Barretina, Jordi, Caponigro, Giordano, Hieronymus, Haley, Murray, Ryan R., Salehi-Ashtiani, Kourosh, Hill, David E., Vidal, Marc, Zhao, Jean J., Yang, Xiaoping, Alkan, Ozan, Kim, Sungjoon, Harris, Jennifer L., Wilson, Christopher J., Myer, Vic E., Finan, Peter M., Root, David E., Roberts, Thomas M., Golub, Todd, Flaherty, Keith T., Dummer, Reinhard, Weber, Barbara, Sellers, William R., Schlegel, Robert, Wargo, Jennifer A., Hahn, William C., Garraway, Levi A.
Published 2010

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies

by Cowley, Glenn S, Weir, Barbara A, Vazquez, Francisca, Tamayo, Pablo, Scott, Justine A, Rusin, Scott, East-Seletsky, Alexandra, Ali, Levi D, Gerath, William F.J, Pantel, Sarah E, Lizotte, Patrick H, Jiang, Guozhi, Hsiao, Jessica, Tsherniak, Aviad, Dwinell, Elizabeth, Aoyama, Simon, Okamoto, Michael, Harrington, William, Gelfand, Ellen, Green, Thomas M, Tomko, Mark J, Gopal, Shuba, Wong, Terence C, Li, Hubo, Howell, Sara, Stransky, Nicolas, Liefeld, Ted, Jang, Dongkeun, Bistline, Jonathan, Meyers, Barbara Hill, Armstrong, Scott A, Anderson, Ken C, Stegmaier, Kimberly, Reich, Michael, Pellman, David, Boehm, Jesse S, Mesirov, Jill P, Golub, Todd R, Root, David E, Hahn, William C
Published 2014

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies

by Cowley, Glenn S, Weir, Barbara A, Vazquez, Francisca, Tamayo, Pablo, Scott, Justine A, Rusin, Scott, East-Seletsky, Alexandra, Ali, Levi D, Gerath, William FJ, Pantel, Sarah E, Lizotte, Patrick H, Jiang, Guozhi, Hsiao, Jessica, Tsherniak, Aviad, Dwinell, Elizabeth, Aoyama, Simon, Okamoto, Michael, Harrington, William, Gelfand, Ellen, Green, Thomas M, Tomko, Mark J, Gopal, Shuba, Wong, Terence C, Li, Hubo, Howell, Sara, Stransky, Nicolas, Liefeld, Ted, Jang, Dongkeun, Bistline, Jonathan, Hill Meyers, Barbara, Armstrong, Scott A, Anderson, Ken C, Stegmaier, Kimberly, Reich, Michael, Pellman, David, Boehm, Jesse S, Mesirov, Jill P, Golub, Todd R, Root, David E, Hahn, William C
Published 2014

Integrated genetic and pharmacologic interrogation of rare cancers

by Hong, Andrew L., Tseng, Yuen-Yi, Cowley, Glenn S., Jonas, Oliver, Cheah, Jaime H., Kynnap, Bryan D., Doshi, Mihir B., Oh, Coyin, Meyer, Stephanie C., Church, Alanna J., Gill, Shubhroz, Bielski, Craig M., Keskula, Paula, Imamovic, Alma, Howell, Sara, Kryukov, Gregory V., Clemons, Paul A., Tsherniak, Aviad, Vazquez, Francisca, Crompton, Brian D., Shamji, Alykhan F., Rodriguez-Galindo, Carlos, Janeway, Katherine A., Roberts, Charles W. M., Stegmaier, Kimberly, van Hummelen, Paul, Cima, Michael J., Langer, Robert S., Garraway, Levi A., Schreiber, Stuart L., Root, David E., Hahn, William C., Boehm, Jesse S.
Published 2016