Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002

Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002

The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperatur...

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Main Authors: Faisal Ali, Anwarali Khan, Awang Ahmad Sallehin, Awang Husaini
Format: Article
Language:English
Published: Asian Network for Scientific network 2006
Subjects:
QD Chemistry
QK Botany
Online Access:http://ir.unimas.my/9817/
http://ir.unimas.my/9817/
http://ir.unimas.my/9817/
http://ir.unimas.my/9817/1/Enhancing%20%C3%83%C5%BD%C3%82%C2%B1-Amylase%20and%20Cellulase%20in%20vivo%20Enzyme%20Expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacilllus%20amyloliquefaciens%20UMAS%201002%20%28ABSRTACT%29.pdf
id unimas-9817
recordtype eprints
spelling unimas-98172015-11-30T00:45:44Z http://ir.unimas.my/9817/ Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002 Faisal Ali, Anwarali Khan Awang Ahmad Sallehin, Awang Husaini QD Chemistry QK Botany The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of α-amylase and cellulase was at 14.19 and 13.15 IU mL <SUP>-1 </SUP>, respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for α-amylase and cellulase, respectively. Enzyme activity was 2.8 (10.80 IU mL <SUP>-1 </SUP>) and 3.2 (9.38 IU mL <SUP>-1 </SUP>) fold higher for α-amylase and cellulase respectively when 1% w/v soluble starch was applied as additive substrates with 0.5% hampas. However in optimal media that consist of 4%w/v of hampas, addition of 1% w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of α-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, Dissolved Oxygen (DO) and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too Asian Network for Scientific network 2006 Article NonPeerReviewed text en http://ir.unimas.my/9817/1/Enhancing%20%C3%83%C5%BD%C3%82%C2%B1-Amylase%20and%20Cellulase%20in%20vivo%20Enzyme%20Expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacilllus%20amyloliquefaciens%20UMAS%201002%20%28ABSRTACT%29.pdf Faisal Ali, Anwarali Khan and Awang Ahmad Sallehin, Awang Husaini (2006) Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002. Biotechnology, 5 (3). pp. 391-403. ISSN 1682-296X http://www.researchgate.net/publication/26556213_Enhancing_-Amylase_and_Cellulase_in_vivo_Enzyme_Expressions_on_Sago_Pith_Residue_Using_Bacilllus_amyloliquefaciens_UMAS_1002 DOI: 10.3923/biotech.2006.391.403
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Sarawak
building UNIMAS Institutional Repository
collection Online Access
language English
topic QD Chemistry
QK Botany
spellingShingle QD Chemistry
QK Botany
Faisal Ali, Anwarali Khan
Awang Ahmad Sallehin, Awang Husaini
Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
description The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of α-amylase and cellulase was at 14.19 and 13.15 IU mL <SUP>-1 </SUP>, respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for α-amylase and cellulase, respectively. Enzyme activity was 2.8 (10.80 IU mL <SUP>-1 </SUP>) and 3.2 (9.38 IU mL <SUP>-1 </SUP>) fold higher for α-amylase and cellulase respectively when 1% w/v soluble starch was applied as additive substrates with 0.5% hampas. However in optimal media that consist of 4%w/v of hampas, addition of 1% w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of α-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, Dissolved Oxygen (DO) and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too
format Article
author Faisal Ali, Anwarali Khan
Awang Ahmad Sallehin, Awang Husaini
author_facet Faisal Ali, Anwarali Khan
Awang Ahmad Sallehin, Awang Husaini
author_sort Faisal Ali, Anwarali Khan
title Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
title_short Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
title_full Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
title_fullStr Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
title_full_unstemmed Enhancing α-Amylase and Cellulase in vivo Enzyme Expressions on Sago Pith Residue Using Bacilllus amyloliquefaciens UMAS 1002
title_sort enhancing ãžâ±-amylase and cellulase in vivo enzyme expressions on sago pith residue using bacilllus amyloliquefaciens umas 1002
publisher Asian Network for Scientific network
publishDate 2006
url http://ir.unimas.my/9817/
http://ir.unimas.my/9817/
http://ir.unimas.my/9817/
http://ir.unimas.my/9817/1/Enhancing%20%C3%83%C5%BD%C3%82%C2%B1-Amylase%20and%20Cellulase%20in%20vivo%20Enzyme%20Expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacilllus%20amyloliquefaciens%20UMAS%201002%20%28ABSRTACT%29.pdf
first_indexed 2018-09-06T15:42:10Z
last_indexed 2018-09-06T15:42:10Z
_version_ 1610873339184676864

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