Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its rela...

Full description

Bibliographic Details
Main Authors:	Hamilton, Eileen P, Kapusta, Aurélie, Huvos, Piroska E, Bidwell, Shelby L, Zafar, Nikhat, Tang, Haibao, Hadjithomas, Michalis, Krishnakumar, Vivek, Badger, Jonathan H, Caler, Elisabet V, Russ, Carsten, Zeng, Qiandong, Fan, Lin, Levin, Joshua Z, Shea, Terrance, Young, Sarah K, Hegarty, Ryan, Daza, Riza, Gujja, Sharvari, Wortman, Jennifer R, Birren, Bruce W, Nusbaum, Chad, Thomas, Jainy, Carey, Clayton M, Pritham, Ellen J, Feschotte, Cédric, Noto, Tomoko, Mochizuki, Kazufumi, Papazyan, Romeo, Taverna, Sean D, Dear, Paul H, Cassidy-Hanley, Donna M, Xiong, Jie, Miao, Wei, Orias, Eduardo, Coyne, Robert S
Format:	Online
Language:	English
Published:	eLife Sciences Publications, Ltd 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5182062/

id	pubmed-5182062
recordtype	oai_dc
spelling	pubmed-51820622016-12-27 Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome Hamilton, Eileen P Kapusta, Aurélie Huvos, Piroska E Bidwell, Shelby L Zafar, Nikhat Tang, Haibao Hadjithomas, Michalis Krishnakumar, Vivek Badger, Jonathan H Caler, Elisabet V Russ, Carsten Zeng, Qiandong Fan, Lin Levin, Joshua Z Shea, Terrance Young, Sarah K Hegarty, Ryan Daza, Riza Gujja, Sharvari Wortman, Jennifer R Birren, Bruce W Nusbaum, Chad Thomas, Jainy Carey, Clayton M Pritham, Ellen J Feschotte, Cédric Noto, Tomoko Mochizuki, Kazufumi Papazyan, Romeo Taverna, Sean D Dear, Paul H Cassidy-Hanley, Donna M Xiong, Jie Miao, Wei Orias, Eduardo Coyne, Robert S Genes and Chromosomes The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymena’s germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum. eLife Sciences Publications, Ltd 2016-11-28 /pmc/articles/PMC5182062/ /pubmed/27892853 http://dx.doi.org/10.7554/eLife.19090 Text en http://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication (http://creativecommons.org/publicdomain/zero/1.0/) .
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hamilton, Eileen P Kapusta, Aurélie Huvos, Piroska E Bidwell, Shelby L Zafar, Nikhat Tang, Haibao Hadjithomas, Michalis Krishnakumar, Vivek Badger, Jonathan H Caler, Elisabet V Russ, Carsten Zeng, Qiandong Fan, Lin Levin, Joshua Z Shea, Terrance Young, Sarah K Hegarty, Ryan Daza, Riza Gujja, Sharvari Wortman, Jennifer R Birren, Bruce W Nusbaum, Chad Thomas, Jainy Carey, Clayton M Pritham, Ellen J Feschotte, Cédric Noto, Tomoko Mochizuki, Kazufumi Papazyan, Romeo Taverna, Sean D Dear, Paul H Cassidy-Hanley, Donna M Xiong, Jie Miao, Wei Orias, Eduardo Coyne, Robert S
spellingShingle	Hamilton, Eileen P Kapusta, Aurélie Huvos, Piroska E Bidwell, Shelby L Zafar, Nikhat Tang, Haibao Hadjithomas, Michalis Krishnakumar, Vivek Badger, Jonathan H Caler, Elisabet V Russ, Carsten Zeng, Qiandong Fan, Lin Levin, Joshua Z Shea, Terrance Young, Sarah K Hegarty, Ryan Daza, Riza Gujja, Sharvari Wortman, Jennifer R Birren, Bruce W Nusbaum, Chad Thomas, Jainy Carey, Clayton M Pritham, Ellen J Feschotte, Cédric Noto, Tomoko Mochizuki, Kazufumi Papazyan, Romeo Taverna, Sean D Dear, Paul H Cassidy-Hanley, Donna M Xiong, Jie Miao, Wei Orias, Eduardo Coyne, Robert S Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
author_facet	Hamilton, Eileen P Kapusta, Aurélie Huvos, Piroska E Bidwell, Shelby L Zafar, Nikhat Tang, Haibao Hadjithomas, Michalis Krishnakumar, Vivek Badger, Jonathan H Caler, Elisabet V Russ, Carsten Zeng, Qiandong Fan, Lin Levin, Joshua Z Shea, Terrance Young, Sarah K Hegarty, Ryan Daza, Riza Gujja, Sharvari Wortman, Jennifer R Birren, Bruce W Nusbaum, Chad Thomas, Jainy Carey, Clayton M Pritham, Ellen J Feschotte, Cédric Noto, Tomoko Mochizuki, Kazufumi Papazyan, Romeo Taverna, Sean D Dear, Paul H Cassidy-Hanley, Donna M Xiong, Jie Miao, Wei Orias, Eduardo Coyne, Robert S
author_sort	Hamilton, Eileen P
title	Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
title_short	Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
title_full	Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
title_fullStr	Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
title_full_unstemmed	Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
title_sort	structure of the germline genome of tetrahymena thermophila and relationship to the massively rearranged somatic genome
description	The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymena’s germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.
publisher	eLife Sciences Publications, Ltd
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5182062/
_version_	1613817220706598912

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Similar Items