Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation

Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation

Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI...

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Main Authors: Brandhorst, Heide, Kurfürst, Manfred, Johnson, Paul R., Korsgren, Olle, Brandhorst, Daniel
Format: Online
Language:English
Published: Lippincott Williams & Wilkins 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946504/
id pubmed-4946504
recordtype oai_dc
spelling pubmed-49465042016-08-05 Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation Brandhorst, Heide Kurfürst, Manfred Johnson, Paul R. Korsgren, Olle Brandhorst, Daniel Pancreas and Islet Transplantation Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI as essential enzymes for human islet isolation. Lippincott Williams & Wilkins 2015-12-15 /pmc/articles/PMC4946504/ /pubmed/27500241 http://dx.doi.org/10.1097/TXD.0000000000000552 Text en Copyright © 2015 The Authors. Transplantation Direct. Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Brandhorst, Heide
Kurfürst, Manfred
Johnson, Paul R.
Korsgren, Olle
Brandhorst, Daniel
spellingShingle Brandhorst, Heide
Kurfürst, Manfred
Johnson, Paul R.
Korsgren, Olle
Brandhorst, Daniel
Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
author_facet Brandhorst, Heide
Kurfürst, Manfred
Johnson, Paul R.
Korsgren, Olle
Brandhorst, Daniel
author_sort Brandhorst, Heide
title Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
title_short Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
title_full Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
title_fullStr Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
title_full_unstemmed Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation
title_sort comparison of neutral proteases and collagenase class i as essential enzymes for human islet isolation
description Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI as essential enzymes for human islet isolation.
publisher Lippincott Williams & Wilkins
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946504/
_version_ 1613609988811390976

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