Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

The interrogation of beta cell gene expression and function in vitro has squarely shifted over the years from the study of rodent tumorigenic cell lines to the study of isolated rodent islets. Primary islets offer the distinct advantage that they more faithfully reflect the biology of intracellular...

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Bibliographic Details
Main Authors: Stull, Natalie D., Breite, Andrew, McCarthy, Robert, Tersey, Sarah A., Mirmira, Raghavendra G.
Format: Online
Language:English
Published: MyJove Corporation 2012
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3490250/