Comparison of Neutral Proteases and Collagenase Class I as Essential Enzymes for Human Islet Isolation

Efficient islet isolation requires synergistic interaction between collagenase class I (CI) and class II (CII). The CI degradation alters the ratio between CI and CII and is responsible for batch-to-batch variations. This study compares the role of neutral protease (NP) plus clostripain (CP) with CI...

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Bibliographic Details
Main Authors: Brandhorst, Heide, Kurfürst, Manfred, Johnson, Paul R., Korsgren, Olle, Brandhorst, Daniel
Format: Online
Language:English
Published: Lippincott Williams & Wilkins 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946504/