Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages

Crotamine is a peptide toxin found in the venom of the rattlesnake Crotalus durissus terrificus and has antiproliferative, antimicrobial, and antifungal activities. Herein, we show that crotamine dose-dependently induced macrophage phagocytic and cytostatic activity by the induction of nitric oxide...

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Main Authors:	Lee, Kyung Jin, Kim, Yun Kyu, Krupa, Martin, Nguyen, Anh Ngoc, Do, Bich Hang, Chung, Boram, Vu, Thi Thu Trang, Kim, Song Cheol, Choe, Han
Format:	Online
Language:	English
Published:	Korean Society for Biochemistry and Molecular Biology 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915234/

id	pubmed-4915234
recordtype	oai_dc
spelling	pubmed-49152342016-06-23 Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages Lee, Kyung Jin Kim, Yun Kyu Krupa, Martin Nguyen, Anh Ngoc Do, Bich Hang Chung, Boram Vu, Thi Thu Trang Kim, Song Cheol Choe, Han Review-Article Crotamine is a peptide toxin found in the venom of the rattlesnake Crotalus durissus terrificus and has antiproliferative, antimicrobial, and antifungal activities. Herein, we show that crotamine dose-dependently induced macrophage phagocytic and cytostatic activity by the induction of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α). Moreover, the crotamineinduced expression of iNOS and TNF-α is mediated through the phosphorylation of p38 and the NF-κB signaling cascade in macrophages. Notably, pretreatment with SB203580 (a p38-specific inhibitor) or BAY 11-7082 (an NF-κB inhibitor) inhibited crotamine-induced NO production and macrophage phagocytic and cytotoxic activity. Our results show for the first time that crotamine stimulates macrophage phagocytic and cytostatic activity by induction of NO and TNF-α via the p38 and NF-κB signaling pathways and suggest that crotamine may be a useful therapeutic agent for the treatment of inflammatory disease. [BMB Reports 2016; 49(3): 185-190] Korean Society for Biochemistry and Molecular Biology 2016-03-31 /pmc/articles/PMC4915234/ /pubmed/26818089 http://dx.doi.org/10.5483/BMBRep.2016.49.3.271 Text en Copyright © 2016, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lee, Kyung Jin Kim, Yun Kyu Krupa, Martin Nguyen, Anh Ngoc Do, Bich Hang Chung, Boram Vu, Thi Thu Trang Kim, Song Cheol Choe, Han
spellingShingle	Lee, Kyung Jin Kim, Yun Kyu Krupa, Martin Nguyen, Anh Ngoc Do, Bich Hang Chung, Boram Vu, Thi Thu Trang Kim, Song Cheol Choe, Han Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
author_facet	Lee, Kyung Jin Kim, Yun Kyu Krupa, Martin Nguyen, Anh Ngoc Do, Bich Hang Chung, Boram Vu, Thi Thu Trang Kim, Song Cheol Choe, Han
author_sort	Lee, Kyung Jin
title	Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
title_short	Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
title_full	Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
title_fullStr	Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
title_full_unstemmed	Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages
title_sort	crotamine stimulates phagocytic activity by inducing nitric oxide and tnf-α via p38 and nfκ-b signaling in raw 264.7 macrophages
description	Crotamine is a peptide toxin found in the venom of the rattlesnake Crotalus durissus terrificus and has antiproliferative, antimicrobial, and antifungal activities. Herein, we show that crotamine dose-dependently induced macrophage phagocytic and cytostatic activity by the induction of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α). Moreover, the crotamineinduced expression of iNOS and TNF-α is mediated through the phosphorylation of p38 and the NF-κB signaling cascade in macrophages. Notably, pretreatment with SB203580 (a p38-specific inhibitor) or BAY 11-7082 (an NF-κB inhibitor) inhibited crotamine-induced NO production and macrophage phagocytic and cytotoxic activity. Our results show for the first time that crotamine stimulates macrophage phagocytic and cytostatic activity by induction of NO and TNF-α via the p38 and NF-κB signaling pathways and suggest that crotamine may be a useful therapeutic agent for the treatment of inflammatory disease. [BMB Reports 2016; 49(3): 185-190]
publisher	Korean Society for Biochemistry and Molecular Biology
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915234/
_version_	1613597548710199296

Crotamine stimulates phagocytic activity by inducing nitric oxide and TNF-α via p38 and NFκ-B signaling in RAW 264.7 macrophages

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