High-performance probes for light and electron microscopy

We describe an engineered family of highly antigenic molecules based on GFP-like fluorescent proteins. These molecules contain numerous copies of peptide epitopes and simultaneously bind IgG antibodies at each location. These “spaghetti monster” fluorescent proteins (smFPs) distribute well in neuron...

Full description

Bibliographic Details
Main Authors: Viswanathan, Sarada, Williams, Megan E., Bloss, Erik B., Stasevich, Timothy J., Speer, Colenso M., Nern, Aljoscha, Pfeiffer, Barret D., Hooks, Bryan M., Li, Wei-Ping, English, Brian P., Tian, Teresa, Henry, Gilbert L., Macklin, John J., Patel, Ronak, Gerfen, Charles R., Zhuang, Xiaowei, Wang, Yalin, Rubin, Gerald M., Looger, Loren L.
Format: Online
Language:English
Published: 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4573404/