Fixation-resistant photoactivatable fluorescent proteins for correlative light and electron microscopy

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they cease to function following heavy fixation, hindering advanced applications such as correlative light and electron microscopy. Here we report engineered variants of the photoconvertible Eos fluore...

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Bibliographic Details
Main Authors: Paez Segala, Maria G., Sun, Mei G., Shtengel, Gleb, Viswanathan, Sarada, Baird, Michelle A., Macklin, John J., Patel, Ronak, Allen, John R., Howe, Elizabeth S., Piszczek, Grzegorz, Hess, Harald F., Davidson, Michael W., Wang, Yalin, Looger, Loren L.
Format: Online
Language:English
Published: 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344411/