A novel inert crystal delivery medium for serial femtosecond crystallography

Viscous sample delivery that decreases the net protein consumed in serial femtosecond crystallography is described. The agarose stream has a low background, is compatible with membrane proteins and can be used at a wide range of temperatures.

Bibliographic Details
Main Authors: Conrad, Chelsie E., Basu, Shibom, James, Daniel, Wang, Dingjie, Schaffer, Alexander, Roy-Chowdhury, Shatabdi, Zatsepin, Nadia A., Aquila, Andrew, Coe, Jesse, Gati, Cornelius, Hunter, Mark S., Koglin, Jason E., Kupitz, Christopher, Nelson, Garrett, Subramanian, Ganesh, White, Thomas A., Zhao, Yun, Zook, James, Boutet, Sébastien, Cherezov, Vadim, Spence, John C. H., Fromme, Raimund, Weierstall, Uwe, Fromme, Petra
Format: Online
Language:English
Published: International Union of Crystallography 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491314/
id pubmed-4491314
recordtype oai_dc
spelling pubmed-44913142015-07-14 A novel inert crystal delivery medium for serial femtosecond crystallography Conrad, Chelsie E. Basu, Shibom James, Daniel Wang, Dingjie Schaffer, Alexander Roy-Chowdhury, Shatabdi Zatsepin, Nadia A. Aquila, Andrew Coe, Jesse Gati, Cornelius Hunter, Mark S. Koglin, Jason E. Kupitz, Christopher Nelson, Garrett Subramanian, Ganesh White, Thomas A. Zhao, Yun Zook, James Boutet, Sébastien Cherezov, Vadim Spence, John C. H. Fromme, Raimund Weierstall, Uwe Fromme, Petra Research Papers Viscous sample delivery that decreases the net protein consumed in serial femtosecond crystallography is described. The agarose stream has a low background, is compatible with membrane proteins and can be used at a wide range of temperatures. International Union of Crystallography 2015-06-30 /pmc/articles/PMC4491314/ /pubmed/26177184 http://dx.doi.org/10.1107/S2052252515009811 Text en © Chelsie E. Conrad et al. 2015 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Conrad, Chelsie E.
Basu, Shibom
James, Daniel
Wang, Dingjie
Schaffer, Alexander
Roy-Chowdhury, Shatabdi
Zatsepin, Nadia A.
Aquila, Andrew
Coe, Jesse
Gati, Cornelius
Hunter, Mark S.
Koglin, Jason E.
Kupitz, Christopher
Nelson, Garrett
Subramanian, Ganesh
White, Thomas A.
Zhao, Yun
Zook, James
Boutet, Sébastien
Cherezov, Vadim
Spence, John C. H.
Fromme, Raimund
Weierstall, Uwe
Fromme, Petra
spellingShingle Conrad, Chelsie E.
Basu, Shibom
James, Daniel
Wang, Dingjie
Schaffer, Alexander
Roy-Chowdhury, Shatabdi
Zatsepin, Nadia A.
Aquila, Andrew
Coe, Jesse
Gati, Cornelius
Hunter, Mark S.
Koglin, Jason E.
Kupitz, Christopher
Nelson, Garrett
Subramanian, Ganesh
White, Thomas A.
Zhao, Yun
Zook, James
Boutet, Sébastien
Cherezov, Vadim
Spence, John C. H.
Fromme, Raimund
Weierstall, Uwe
Fromme, Petra
A novel inert crystal delivery medium for serial femtosecond crystallography
author_facet Conrad, Chelsie E.
Basu, Shibom
James, Daniel
Wang, Dingjie
Schaffer, Alexander
Roy-Chowdhury, Shatabdi
Zatsepin, Nadia A.
Aquila, Andrew
Coe, Jesse
Gati, Cornelius
Hunter, Mark S.
Koglin, Jason E.
Kupitz, Christopher
Nelson, Garrett
Subramanian, Ganesh
White, Thomas A.
Zhao, Yun
Zook, James
Boutet, Sébastien
Cherezov, Vadim
Spence, John C. H.
Fromme, Raimund
Weierstall, Uwe
Fromme, Petra
author_sort Conrad, Chelsie E.
title A novel inert crystal delivery medium for serial femtosecond crystallography
title_short A novel inert crystal delivery medium for serial femtosecond crystallography
title_full A novel inert crystal delivery medium for serial femtosecond crystallography
title_fullStr A novel inert crystal delivery medium for serial femtosecond crystallography
title_full_unstemmed A novel inert crystal delivery medium for serial femtosecond crystallography
title_sort novel inert crystal delivery medium for serial femtosecond crystallography
description Viscous sample delivery that decreases the net protein consumed in serial femtosecond crystallography is described. The agarose stream has a low background, is compatible with membrane proteins and can be used at a wide range of temperatures.
publisher International Union of Crystallography
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491314/
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