A novel inert crystal delivery medium for serial femtosecond crystallography

Viscous sample delivery that decreases the net protein consumed in serial femtosecond crystallography is described. The agarose stream has a low background, is compatible with membrane proteins and can be used at a wide range of temperatures.

Bibliographic Details
Main Authors: Conrad, Chelsie E., Basu, Shibom, James, Daniel, Wang, Dingjie, Schaffer, Alexander, Roy-Chowdhury, Shatabdi, Zatsepin, Nadia A., Aquila, Andrew, Coe, Jesse, Gati, Cornelius, Hunter, Mark S., Koglin, Jason E., Kupitz, Christopher, Nelson, Garrett, Subramanian, Ganesh, White, Thomas A., Zhao, Yun, Zook, James, Boutet, Sébastien, Cherezov, Vadim, Spence, John C. H., Fromme, Raimund, Weierstall, Uwe, Fromme, Petra
Format: Online
Language:English
Published: International Union of Crystallography 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491314/