Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons
Mutation of the human gene superoxide dismutase (hSOD1) is associated with the fatal neurodegenerative disease familial amyotrophic lateral sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1 in Drosophila motorneurons increases lifespan to 140% of normal. The current study was desig...
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Sociedade Brasileira de Genética
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415569/ |
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pubmed-44155692015-05-15 Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons Petrosyan, Agavni Hsieh, I-Hui Phillips, John P. Saberi, Kourosh Cellular, Molecular and Developmental Genetics Mutation of the human gene superoxide dismutase (hSOD1) is associated with the fatal neurodegenerative disease familial amyotrophic lateral sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1 in Drosophila motorneurons increases lifespan to 140% of normal. The current study was designed to determine resistance to lifespan decline and failure of sensorimotor functions by overexpressing hSOD1 in Drosophila‘s motorneurons. First, we measured the ability to maintain continuous flight and wingbeat frequency (WBF) as a function of age (5 to 50 days). Flies overexpressing hSOD1 under the D42-GAL4 activator were able to sustain flight significantly longer than controls, with the largest effect observed in the middle stages of life. The hSOD1-expressed line also had, on average, slower wingbeat frequencies in late, but not early life relative to age-matched controls. Second, we examined locomotor (exploratory walking) behavior in late life when flies had lost the ability to fly (age ≥ 60 d). hSOD1-expressed flies showed significantly more robust walking activity relative to controls. Findings show patterns of functional decline dissimilar to those reported for other life-extended lines, and suggest that the hSOD1 gene not only delays death but enhances sensorimotor abilities critical to survival even in late life. Sociedade Brasileira de Genética 2015-03 2014-03-17 /pmc/articles/PMC4415569/ /pubmed/25983632 http://dx.doi.org/10.1590/S1415-475738138120140132 Text en Copyright © 2015, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Petrosyan, Agavni Hsieh, I-Hui Phillips, John P. Saberi, Kourosh |
| spellingShingle |
Petrosyan, Agavni Hsieh, I-Hui Phillips, John P. Saberi, Kourosh Enhanced tethered-flight duration and locomotor activity by overexpression of the human gene SOD1 in Drosophila motorneurons |
| author_facet |
Petrosyan, Agavni Hsieh, I-Hui Phillips, John P. Saberi, Kourosh |
| author_sort |
Petrosyan, Agavni |
| title |
Enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene SOD1 in Drosophila
motorneurons |
| title_short |
Enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene SOD1 in Drosophila
motorneurons |
| title_full |
Enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene SOD1 in Drosophila
motorneurons |
| title_fullStr |
Enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene SOD1 in Drosophila
motorneurons |
| title_full_unstemmed |
Enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene SOD1 in Drosophila
motorneurons |
| title_sort |
enhanced tethered-flight duration and locomotor activity by
overexpression of the human gene sod1 in drosophila
motorneurons |
| description |
Mutation of the human gene superoxide dismutase (hSOD1) is
associated with the fatal neurodegenerative disease familial amyotrophic lateral
sclerosis (Lou Gehrig’s disease). Selective overexpression of hSOD1
in Drosophila motorneurons increases lifespan to 140% of normal. The
current study was designed to determine resistance to lifespan decline and failure of
sensorimotor functions by overexpressing hSOD1 in
Drosophila‘s motorneurons. First, we measured the ability to
maintain continuous flight and wingbeat frequency (WBF) as a function of age (5 to 50
days). Flies overexpressing hSOD1 under the D42-GAL4 activator were
able to sustain flight significantly longer than controls, with the largest effect
observed in the middle stages of life. The hSOD1-expressed line also
had, on average, slower wingbeat frequencies in late, but not early life relative to
age-matched controls. Second, we examined locomotor (exploratory walking) behavior in
late life when flies had lost the ability to fly (age ≥ 60 d).
hSOD1-expressed flies showed significantly more robust walking
activity relative to controls. Findings show patterns of functional decline
dissimilar to those reported for other life-extended lines, and suggest that the
hSOD1 gene not only delays death but enhances sensorimotor
abilities critical to survival even in late life. |
| publisher |
Sociedade Brasileira de Genética |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4415569/ |
| _version_ |
1613217827037118464 |