Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans

Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans

The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been successfully used for genome editing in a variety of organisms. Here, we report the use of dual sgRNA-guided Cas9 nuclease to generate knockout mutants of protein coding genes, noncoding genes, and repetitive sequences in C. elegans....

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Bibliographic Details
Main Authors: Chen, Xiangyang, Xu, Fei, Zhu, Chengming, Ji, Jiaojiao, Zhou, Xufei, Feng, Xuezhu, Guang, Shouhong
Format: Online
Language:English
Published: Nature Publishing Group 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273605/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4273605/

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