Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency

CRISPR-Cas9 is a powerful genome editing technology, yet with off-target effects. Truncated sgRNAs (17nt) have been found to decrease off-target cleavage without affecting on-target disruption in 293T cells. However, the potency of 17nt sgRNAs relative to the full-length 20nt sgRNAs in stem cells, s...

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Bibliographic Details
Main Authors: Zhang, Jian-Ping, Li, Xiao-Lan, Neises, Amanda, Chen, Wanqiu, Hu, Lin-Ping, Ji, Guang-Zhen, Yu, Jun-Yao, Xu, Jing, Yuan, Wei-Ping, Cheng, Tao, Zhang, Xiao-Bing
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919781/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919781/

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