Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites

Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance li...

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Main Authors:	Trammell, Samuel AJ, Brenner, Charles
Format:	Online
Language:	English
Published:	Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962138/

id	pubmed-3962138
recordtype	oai_dc
spelling	pubmed-39621382014-03-31 Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites Trammell, Samuel AJ Brenner, Charles Mini Reviews Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds. Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2013-05-27 /pmc/articles/PMC3962138/ /pubmed/24688693 http://dx.doi.org/10.5936/csbj.201301012 Text en © Trammell and Brenner. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Trammell, Samuel AJ Brenner, Charles
spellingShingle	Trammell, Samuel AJ Brenner, Charles Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
author_facet	Trammell, Samuel AJ Brenner, Charles
author_sort	Trammell, Samuel AJ
title	Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
title_short	Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
title_full	Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
title_fullStr	Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
title_full_unstemmed	Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites
title_sort	targeted, lcms-based metabolomics for quantitative measurement of nad+ metabolites
description	Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.
publisher	Research Network of Computational and Structural Biotechnology (RNCSB) Organization
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962138/
_version_	1612070238801625088

Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD+ Metabolites

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