Accelerated growth in the absence of DNA replication origins
DNA replication initiates at defined sites called origins, which serve as binding sites for initiator proteins that recruit the replicative machinery. Origins differ in number and structure across the three domains of life1 and their properties determine the dynamics of chromosome replication. Bacte...
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2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843117/ |
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pubmed-38431172014-05-28 Accelerated growth in the absence of DNA replication origins Hawkins, Michelle Malla, Sunir Blythe, Martin J. Nieduszynski, Conrad A. Allers, Thorsten Article DNA replication initiates at defined sites called origins, which serve as binding sites for initiator proteins that recruit the replicative machinery. Origins differ in number and structure across the three domains of life1 and their properties determine the dynamics of chromosome replication. Bacteria and some archaea replicate from single origins, whilst most archaea and all eukaryotes replicate using multiple origins. Initiation mechanisms that rely on homologous recombination operate in some viruses. Here we show that such mechanisms also operate in archaea. We have used deep sequencing to study replication in Haloferax volcanii. Four chromosomal origins of differing activity were identified. Deletion of individual origins resulted in perturbed replication dynamics and reduced growth. However, a strain lacking all origins has no apparent defects and grows significantly faster than wild-type. Origin-less cells initiate replication at dispersed sites rather than at discrete origins and have an absolute requirement for the recombinase RadA, unlike strains lacking individual origins. Our results demonstrate that homologous recombination alone can efficiently initiate the replication of an entire cellular genome. This raises the question of what purpose replication origins serve and why they have evolved. 2013-11-03 2013-11-28 /pmc/articles/PMC3843117/ /pubmed/24185008 http://dx.doi.org/10.1038/nature12650 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Hawkins, Michelle Malla, Sunir Blythe, Martin J. Nieduszynski, Conrad A. Allers, Thorsten |
| spellingShingle |
Hawkins, Michelle Malla, Sunir Blythe, Martin J. Nieduszynski, Conrad A. Allers, Thorsten Accelerated growth in the absence of DNA replication origins |
| author_facet |
Hawkins, Michelle Malla, Sunir Blythe, Martin J. Nieduszynski, Conrad A. Allers, Thorsten |
| author_sort |
Hawkins, Michelle |
| title |
Accelerated growth in the absence of DNA replication origins |
| title_short |
Accelerated growth in the absence of DNA replication origins |
| title_full |
Accelerated growth in the absence of DNA replication origins |
| title_fullStr |
Accelerated growth in the absence of DNA replication origins |
| title_full_unstemmed |
Accelerated growth in the absence of DNA replication origins |
| title_sort |
accelerated growth in the absence of dna replication origins |
| description |
DNA replication initiates at defined sites called origins, which serve as binding sites for initiator proteins that recruit the replicative machinery. Origins differ in number and structure across the three domains of life1 and their properties determine the dynamics of chromosome replication. Bacteria and some archaea replicate from single origins, whilst most archaea and all eukaryotes replicate using multiple origins. Initiation mechanisms that rely on homologous recombination operate in some viruses. Here we show that such mechanisms also operate in archaea. We have used deep sequencing to study replication in Haloferax volcanii. Four chromosomal origins of differing activity were identified. Deletion of individual origins resulted in perturbed replication dynamics and reduced growth. However, a strain lacking all origins has no apparent defects and grows significantly faster than wild-type. Origin-less cells initiate replication at dispersed sites rather than at discrete origins and have an absolute requirement for the recombinase RadA, unlike strains lacking individual origins. Our results demonstrate that homologous recombination alone can efficiently initiate the replication of an entire cellular genome. This raises the question of what purpose replication origins serve and why they have evolved. |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3843117/ |
| _version_ |
1612031602102108160 |