Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease

Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protects against oxidative stress which is important in the pathogenesis of chronic obstructive pulmonary disease (COPD). Three single nucleotide polymorphisms and 1 triplet repeat polymorphism are found in the promoter region of the Nrf2 gene. Mole...

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Main Authors:	Hua, Chung-Ching, Chang, Liang-Che, Tseng, Jo-Chi, Chu, Chien-Ming, Liu, Yu-Chih, Shieh, Wen-Bin
Format:	Online
Language:	English
Published:	IOS Press 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833611/

id	pubmed-3833611
recordtype	oai_dc
spelling	pubmed-38336112013-12-17 Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease Hua, Chung-Ching Chang, Liang-Che Tseng, Jo-Chi Chu, Chien-Ming Liu, Yu-Chih Shieh, Wen-Bin Other Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protects against oxidative stress which is important in the pathogenesis of chronic obstructive pulmonary disease (COPD). Three single nucleotide polymorphisms and 1 triplet repeat polymorphism are found in the promoter region of the Nrf2 gene. Molecular haplotyping of the Nrf2 promoter region was performed using DNA obtained from the peripheral blood of 69 COPD patients. The luciferase activities of Nrf2 promoter constructs containing all possible combinations of the 4 polymorphisms were determined and found to differ among the 16 haplotypes.The haplotypes isolated from the subjects were divided into 3 groups (L: low; M: medium; H: high) on the basis of luciferase activities. The proportions of subjects belonging to global initiative for chronic obstructive lung disease stage 3 or 4 decreased from the group with the LL haplotype to that with the HH haplotype. Presence of the LH or MM haplotype (hazard ratio, 3.36; 95% confidence interval, 1.16–9.69), gender (0.13; 0.02–0.67), and post-bronchodilator FEV1 value of predicted (0.95; 0.91–0.99) are significant predictors of respiratory failure development.The haplotype of the Nrf2 gene promoter affects its activity, and is associated with the severity and the development of respiratory failure in COPD. IOS Press 2010 2010-06-09 /pmc/articles/PMC3833611/ /pubmed/20534903 http://dx.doi.org/10.3233/DMA-2010-0700 Text en Copyright © 2010 Hindawi Publishing Corporation.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Hua, Chung-Ching Chang, Liang-Che Tseng, Jo-Chi Chu, Chien-Ming Liu, Yu-Chih Shieh, Wen-Bin
spellingShingle	Hua, Chung-Ching Chang, Liang-Che Tseng, Jo-Chi Chu, Chien-Ming Liu, Yu-Chih Shieh, Wen-Bin Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
author_facet	Hua, Chung-Ching Chang, Liang-Che Tseng, Jo-Chi Chu, Chien-Ming Liu, Yu-Chih Shieh, Wen-Bin
author_sort	Hua, Chung-Ching
title	Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
title_short	Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
title_full	Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
title_fullStr	Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
title_full_unstemmed	Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease
title_sort	functional haplotypes in the promoter region of transcription factor nrf2 in chronic obstructive pulmonary disease
description	Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) protects against oxidative stress which is important in the pathogenesis of chronic obstructive pulmonary disease (COPD). Three single nucleotide polymorphisms and 1 triplet repeat polymorphism are found in the promoter region of the Nrf2 gene. Molecular haplotyping of the Nrf2 promoter region was performed using DNA obtained from the peripheral blood of 69 COPD patients. The luciferase activities of Nrf2 promoter constructs containing all possible combinations of the 4 polymorphisms were determined and found to differ among the 16 haplotypes.The haplotypes isolated from the subjects were divided into 3 groups (L: low; M: medium; H: high) on the basis of luciferase activities. The proportions of subjects belonging to global initiative for chronic obstructive lung disease stage 3 or 4 decreased from the group with the LL haplotype to that with the HH haplotype. Presence of the LH or MM haplotype (hazard ratio, 3.36; 95% confidence interval, 1.16–9.69), gender (0.13; 0.02–0.67), and post-bronchodilator FEV1 value of predicted (0.95; 0.91–0.99) are significant predictors of respiratory failure development.The haplotype of the Nrf2 gene promoter affects its activity, and is associated with the severity and the development of respiratory failure in COPD.
publisher	IOS Press
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833611/
_version_	1612028549582028800

Functional Haplotypes in the Promoter Region of Transcription Factor Nrf2 in Chronic Obstructive Pulmonary Disease

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