Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection

Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis of a complete reference genome and 81 draft sequences from clonal complex 180, the predominant serotype 3 clone in much of the...

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Main Authors:	Croucher, Nicholas J., Mitchell, Andrea M., Gould, Katherine A., Inverarity, Donald, Barquist, Lars, Feltwell, Theresa, Fookes, Maria C., Harris, Simon R., Dordel, Janina, Salter, Susannah J., Browall, Sarah, Zemlickova, Helena, Parkhill, Julian, Normark, Staffan, Henriques-Normark, Birgitta, Hinds, Jason, Mitchell, Tim J., Bentley, Stephen D.
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794909/

id	pubmed-3794909
recordtype	oai_dc
spelling	pubmed-37949092013-10-15 Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection Croucher, Nicholas J. Mitchell, Andrea M. Gould, Katherine A. Inverarity, Donald Barquist, Lars Feltwell, Theresa Fookes, Maria C. Harris, Simon R. Dordel, Janina Salter, Susannah J. Browall, Sarah Zemlickova, Helena Parkhill, Julian Normark, Staffan Henriques-Normark, Birgitta Hinds, Jason Mitchell, Tim J. Bentley, Stephen D. Research Article Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis of a complete reference genome and 81 draft sequences from clonal complex 180, the predominant serotype 3 clone in much of the world, found most sampled isolates belonged to a clade affected by few diversifying recombinations. However, other isolates indicate significant genetic variation has accumulated over the clonal complex's entire history. Two closely related genomes, one from the blood and another from the cerebrospinal fluid, were obtained from a patient with meningitis. The pair differed in their behaviour in a mouse model of disease and in their susceptibility to antimicrobials, with at least some of these changes attributable to a mutation that up-regulated the patAB efflux pump. This indicates clinically important phenotypic variation can accumulate rapidly through small alterations to the genotype. Public Library of Science 2013-10-10 /pmc/articles/PMC3794909/ /pubmed/24130509 http://dx.doi.org/10.1371/journal.pgen.1003868 Text en © 2013 Croucher et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Croucher, Nicholas J. Mitchell, Andrea M. Gould, Katherine A. Inverarity, Donald Barquist, Lars Feltwell, Theresa Fookes, Maria C. Harris, Simon R. Dordel, Janina Salter, Susannah J. Browall, Sarah Zemlickova, Helena Parkhill, Julian Normark, Staffan Henriques-Normark, Birgitta Hinds, Jason Mitchell, Tim J. Bentley, Stephen D.
spellingShingle	Croucher, Nicholas J. Mitchell, Andrea M. Gould, Katherine A. Inverarity, Donald Barquist, Lars Feltwell, Theresa Fookes, Maria C. Harris, Simon R. Dordel, Janina Salter, Susannah J. Browall, Sarah Zemlickova, Helena Parkhill, Julian Normark, Staffan Henriques-Normark, Birgitta Hinds, Jason Mitchell, Tim J. Bentley, Stephen D. Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
author_facet	Croucher, Nicholas J. Mitchell, Andrea M. Gould, Katherine A. Inverarity, Donald Barquist, Lars Feltwell, Theresa Fookes, Maria C. Harris, Simon R. Dordel, Janina Salter, Susannah J. Browall, Sarah Zemlickova, Helena Parkhill, Julian Normark, Staffan Henriques-Normark, Birgitta Hinds, Jason Mitchell, Tim J. Bentley, Stephen D.
author_sort	Croucher, Nicholas J.
title	Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
title_short	Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
title_full	Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
title_fullStr	Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
title_full_unstemmed	Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection
title_sort	dominant role of nucleotide substitution in the diversification of serotype 3 pneumococci over decades and during a single infection
description	Streptococcus pneumoniae of serotype 3 possess a mucoid capsule and cause disease associated with high mortality rates relative to other pneumococci. Phylogenetic analysis of a complete reference genome and 81 draft sequences from clonal complex 180, the predominant serotype 3 clone in much of the world, found most sampled isolates belonged to a clade affected by few diversifying recombinations. However, other isolates indicate significant genetic variation has accumulated over the clonal complex's entire history. Two closely related genomes, one from the blood and another from the cerebrospinal fluid, were obtained from a patient with meningitis. The pair differed in their behaviour in a mouse model of disease and in their susceptibility to antimicrobials, with at least some of these changes attributable to a mutation that up-regulated the patAB efflux pump. This indicates clinically important phenotypic variation can accumulate rapidly through small alterations to the genotype.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794909/
_version_	1612017739144101888

Dominant Role of Nucleotide Substitution in the Diversification of Serotype 3 Pneumococci over Decades and during a Single Infection

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