Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens
Defense against attaching and effacing (A/E) bacteria requires the sequential generation of interleukin 23 (IL-23) and IL-22 to induce protective mucosal responses. While CD4+ and NKp46+ innate lymphoid cells (ILCs) are the critical source of IL-22 during infection, the precise source of IL-23 is un...
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| Format: | Online |
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2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788683/ |
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pubmed-37886832014-03-01 Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens Satpathy, Ansuman T. Briseño, Carlos G. Lee, Jacob S. Ng, Dennis Manieri, Nicholas A. KC, Wumesh Wu, Xiaodi Thomas, Stephanie R. Lee, Wan-Ling Turkoz, Mustafa McDonald, Keely G. Meredith, Matthew M. Song, Christina Guidos, Cynthia J. Newberry, Rodney D. Ouyang, Wenjun Murphy, Theresa L. Stappenbeck, Thaddeus S. Gommerman, Jennifer L. Nussenzweig, Michel C. Colonna, Marco Kopan, Raphael Murphy, Kenneth M. Article Defense against attaching and effacing (A/E) bacteria requires the sequential generation of interleukin 23 (IL-23) and IL-22 to induce protective mucosal responses. While CD4+ and NKp46+ innate lymphoid cells (ILCs) are the critical source of IL-22 during infection, the precise source of IL-23 is unclear. We used genetic techniques to deplete specific subsets of classical dendritic cells (cDCs) and analyzed immunity to the A/E pathogen Citrobacter rodentium. We found that Notch2 controlled the terminal stage of cDC differentiation. Notch2-dependent intestinal CD11b+ cDCs, but not Batf3-dependent CD103+ cDCs, were an obligate source of IL-23 required to survive C. rodentium infection. These results provide the first demonstration of a non-redundant function of CD11b+ cDCs in response to pathogens in vivo. 2013-08-04 2013-09 /pmc/articles/PMC3788683/ /pubmed/23913046 http://dx.doi.org/10.1038/ni.2679 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Satpathy, Ansuman T. Briseño, Carlos G. Lee, Jacob S. Ng, Dennis Manieri, Nicholas A. KC, Wumesh Wu, Xiaodi Thomas, Stephanie R. Lee, Wan-Ling Turkoz, Mustafa McDonald, Keely G. Meredith, Matthew M. Song, Christina Guidos, Cynthia J. Newberry, Rodney D. Ouyang, Wenjun Murphy, Theresa L. Stappenbeck, Thaddeus S. Gommerman, Jennifer L. Nussenzweig, Michel C. Colonna, Marco Kopan, Raphael Murphy, Kenneth M. |
| spellingShingle |
Satpathy, Ansuman T. Briseño, Carlos G. Lee, Jacob S. Ng, Dennis Manieri, Nicholas A. KC, Wumesh Wu, Xiaodi Thomas, Stephanie R. Lee, Wan-Ling Turkoz, Mustafa McDonald, Keely G. Meredith, Matthew M. Song, Christina Guidos, Cynthia J. Newberry, Rodney D. Ouyang, Wenjun Murphy, Theresa L. Stappenbeck, Thaddeus S. Gommerman, Jennifer L. Nussenzweig, Michel C. Colonna, Marco Kopan, Raphael Murphy, Kenneth M. Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| author_facet |
Satpathy, Ansuman T. Briseño, Carlos G. Lee, Jacob S. Ng, Dennis Manieri, Nicholas A. KC, Wumesh Wu, Xiaodi Thomas, Stephanie R. Lee, Wan-Ling Turkoz, Mustafa McDonald, Keely G. Meredith, Matthew M. Song, Christina Guidos, Cynthia J. Newberry, Rodney D. Ouyang, Wenjun Murphy, Theresa L. Stappenbeck, Thaddeus S. Gommerman, Jennifer L. Nussenzweig, Michel C. Colonna, Marco Kopan, Raphael Murphy, Kenneth M. |
| author_sort |
Satpathy, Ansuman T. |
| title |
Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| title_short |
Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| title_full |
Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| title_fullStr |
Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| title_full_unstemmed |
Notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| title_sort |
notch2-dependent classical dendritic cells orchestrate intestinal immunity against attaching and effacing bacterial pathogens |
| description |
Defense against attaching and effacing (A/E) bacteria requires the sequential generation of interleukin 23 (IL-23) and IL-22 to induce protective mucosal responses. While CD4+ and NKp46+ innate lymphoid cells (ILCs) are the critical source of IL-22 during infection, the precise source of IL-23 is unclear. We used genetic techniques to deplete specific subsets of classical dendritic cells (cDCs) and analyzed immunity to the A/E pathogen Citrobacter rodentium. We found that Notch2 controlled the terminal stage of cDC differentiation. Notch2-dependent intestinal CD11b+ cDCs, but not Batf3-dependent CD103+ cDCs, were an obligate source of IL-23 required to survive C. rodentium infection. These results provide the first demonstration of a non-redundant function of CD11b+ cDCs in response to pathogens in vivo. |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788683/ |
| _version_ |
1612015734353821696 |