Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles
Connexin36 (Cx36), a trans-membrane protein that forms gap junctions between insulin-secreting beta-cells in the Langerhans islets, contributes to the proper control of insulin secretion and beta-cell survival. Hypercholesterolemia and pro-atherogenic low density lipoproteins (LDL) contribute to bet...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559396/ |
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pubmed-35593962013-02-04 Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles Haefliger, Jacques-Antoine Martin, David Favre, Dimitri Petremand, Yannick Mazzolai, Lucia Abderrahmani, Amar Meda, Paolo Waeber, Gérard Allagnat, Florent Research Article Connexin36 (Cx36), a trans-membrane protein that forms gap junctions between insulin-secreting beta-cells in the Langerhans islets, contributes to the proper control of insulin secretion and beta-cell survival. Hypercholesterolemia and pro-atherogenic low density lipoproteins (LDL) contribute to beta-cell dysfunction and apoptosis in the context of Type 2 diabetes. We investigated the impact of LDL-cholesterol on Cx36 levels in beta-cells. As compared to WT mice, the Cx36 content was reduced in islets from hypercholesterolemic ApoE−/− mice. Prolonged exposure to human native (nLDL) or oxidized LDL (oxLDL) particles decreased the expression of Cx36 in insulin secreting cell-lines and isolated rodent islets. Cx36 down-regulation was associated with overexpression of the inducible cAMP early repressor (ICER-1) and the selective disruption of ICER-1 prevented the effects of oxLDL on Cx36 expression. Oil red O staining and Plin1 expression levels suggested that oxLDL were less stored as neutral lipid droplets than nLDL in INS-1E cells. The lipid beta-oxidation inhibitor etomoxir enhanced oxLDL-induced apoptosis whereas the ceramide synthesis inhibitor myriocin partially protected INS-1E cells, suggesting that oxLDL toxicity was due to impaired metabolism of the lipids. ICER-1 and Cx36 expressions were closely correlated with oxLDL toxicity. Cx36 knock-down in INS-1E cells or knock-out in primary islets sensitized beta-cells to oxLDL-induced apoptosis. In contrast, overexpression of Cx36 partially protected INS-1E cells against apoptosis. These data demonstrate that the reduction of Cx36 content in beta-cells by oxLDL particles is mediated by ICER-1 and contributes to oxLDL-induced beta-cell apoptosis. Public Library of Science 2013-01-30 /pmc/articles/PMC3559396/ /pubmed/23383107 http://dx.doi.org/10.1371/journal.pone.0055198 Text en © 2013 Haefliger et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Haefliger, Jacques-Antoine Martin, David Favre, Dimitri Petremand, Yannick Mazzolai, Lucia Abderrahmani, Amar Meda, Paolo Waeber, Gérard Allagnat, Florent |
| spellingShingle |
Haefliger, Jacques-Antoine Martin, David Favre, Dimitri Petremand, Yannick Mazzolai, Lucia Abderrahmani, Amar Meda, Paolo Waeber, Gérard Allagnat, Florent Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| author_facet |
Haefliger, Jacques-Antoine Martin, David Favre, Dimitri Petremand, Yannick Mazzolai, Lucia Abderrahmani, Amar Meda, Paolo Waeber, Gérard Allagnat, Florent |
| author_sort |
Haefliger, Jacques-Antoine |
| title |
Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| title_short |
Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| title_full |
Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| title_fullStr |
Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| title_full_unstemmed |
Reduction of Connexin36 Content by ICER-1 Contributes to Insulin-Secreting Cells Apoptosis Induced by Oxidized LDL Particles |
| title_sort |
reduction of connexin36 content by icer-1 contributes to insulin-secreting cells apoptosis induced by oxidized ldl particles |
| description |
Connexin36 (Cx36), a trans-membrane protein that forms gap junctions between insulin-secreting beta-cells in the Langerhans islets, contributes to the proper control of insulin secretion and beta-cell survival. Hypercholesterolemia and pro-atherogenic low density lipoproteins (LDL) contribute to beta-cell dysfunction and apoptosis in the context of Type 2 diabetes. We investigated the impact of LDL-cholesterol on Cx36 levels in beta-cells. As compared to WT mice, the Cx36 content was reduced in islets from hypercholesterolemic ApoE−/− mice. Prolonged exposure to human native (nLDL) or oxidized LDL (oxLDL) particles decreased the expression of Cx36 in insulin secreting cell-lines and isolated rodent islets. Cx36 down-regulation was associated with overexpression of the inducible cAMP early repressor (ICER-1) and the selective disruption of ICER-1 prevented the effects of oxLDL on Cx36 expression. Oil red O staining and Plin1 expression levels suggested that oxLDL were less stored as neutral lipid droplets than nLDL in INS-1E cells. The lipid beta-oxidation inhibitor etomoxir enhanced oxLDL-induced apoptosis whereas the ceramide synthesis inhibitor myriocin partially protected INS-1E cells, suggesting that oxLDL toxicity was due to impaired metabolism of the lipids. ICER-1 and Cx36 expressions were closely correlated with oxLDL toxicity. Cx36 knock-down in INS-1E cells or knock-out in primary islets sensitized beta-cells to oxLDL-induced apoptosis. In contrast, overexpression of Cx36 partially protected INS-1E cells against apoptosis. These data demonstrate that the reduction of Cx36 content in beta-cells by oxLDL particles is mediated by ICER-1 and contributes to oxLDL-induced beta-cell apoptosis. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3559396/ |
| _version_ |
1611950906023084032 |