Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases
Telomeric sequences are added by an enzyme called telomerase that is made of two components: a catalytic protein called telomerase reverse transcriptase (TERT) and an integral RNA template (TR). Telomerase expression is tightly regulated at each step of gene expression, including alternative splicin...
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| Format: | Online |
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Public Library of Science
2012
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480478/ |
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pubmed-3480478 |
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pubmed-34804782012-10-29 Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases Withers, Johanna B. Ashvetiya, Tamara Beemon, Karen L. Research Article Telomeric sequences are added by an enzyme called telomerase that is made of two components: a catalytic protein called telomerase reverse transcriptase (TERT) and an integral RNA template (TR). Telomerase expression is tightly regulated at each step of gene expression, including alternative splicing of TERT mRNA. While over a dozen different alternative splicing events have been reported for human TERT mRNA, these were all in the 3′ half of the coding region. We were interested in examining splicing of the 5′ half of hTERT mRNA, especially since exon 2 is unusually large (1.3 kb). Internal mammalian exons are usually short, typically only 50 to 300 nucleotides, and most long internal exons are alternatively processed. We used quantitative RT-PCR and high-throughput sequencing data to examine the variety and quantity of mRNA species generated from the hTERT locus. We determined that there are approximately 20–40 molecules of hTERT mRNA per cell in the A431 human cell line. In addition, we describe an abundant, alternatively-spliced mRNA variant that excludes TERT exon 2 and was seen in other primates. This variant causes a frameshift and results in translation termination in exon 3, generating a 12 kDa polypeptide. Public Library of Science 2012-10-24 /pmc/articles/PMC3480478/ /pubmed/23110161 http://dx.doi.org/10.1371/journal.pone.0048016 Text en © 2012 Withers et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Withers, Johanna B. Ashvetiya, Tamara Beemon, Karen L. |
| spellingShingle |
Withers, Johanna B. Ashvetiya, Tamara Beemon, Karen L. Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| author_facet |
Withers, Johanna B. Ashvetiya, Tamara Beemon, Karen L. |
| author_sort |
Withers, Johanna B. |
| title |
Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| title_short |
Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| title_full |
Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| title_fullStr |
Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| title_full_unstemmed |
Exclusion of Exon 2 Is a Common mRNA Splice Variant of Primate Telomerase Reverse Transcriptases |
| title_sort |
exclusion of exon 2 is a common mrna splice variant of primate telomerase reverse transcriptases |
| description |
Telomeric sequences are added by an enzyme called telomerase that is made of two components: a catalytic protein called telomerase reverse transcriptase (TERT) and an integral RNA template (TR). Telomerase expression is tightly regulated at each step of gene expression, including alternative splicing of TERT mRNA. While over a dozen different alternative splicing events have been reported for human TERT mRNA, these were all in the 3′ half of the coding region. We were interested in examining splicing of the 5′ half of hTERT mRNA, especially since exon 2 is unusually large (1.3 kb). Internal mammalian exons are usually short, typically only 50 to 300 nucleotides, and most long internal exons are alternatively processed. We used quantitative RT-PCR and high-throughput sequencing data to examine the variety and quantity of mRNA species generated from the hTERT locus. We determined that there are approximately 20–40 molecules of hTERT mRNA per cell in the A431 human cell line. In addition, we describe an abundant, alternatively-spliced mRNA variant that excludes TERT exon 2 and was seen in other primates. This variant causes a frameshift and results in translation termination in exon 3, generating a 12 kDa polypeptide. |
| publisher |
Public Library of Science |
| publishDate |
2012 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480478/ |
| _version_ |
1611918658645262336 |