Bayesian localisation microscopy reveals nanoscale podosome dynamics

We demonstrate a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and Xenon arc lamp illumination. Our Bayesian analysis of blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated...

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Main Authors:	Cox, Susan, Rosten, Edward, Monypenny, James, Jovanovic-Talisman, Tijana, Burnette, Dylan T., Lippincott-Schwartz, Jennifer, Jones, Gareth E., Heintzmann, Rainer
Format:	Online
Language:	English
Published:	2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272474/

id	pubmed-3272474
recordtype	oai_dc
spelling	pubmed-32724742012-08-01 Bayesian localisation microscopy reveals nanoscale podosome dynamics Cox, Susan Rosten, Edward Monypenny, James Jovanovic-Talisman, Tijana Burnette, Dylan T. Lippincott-Schwartz, Jennifer Jones, Gareth E. Heintzmann, Rainer Article We demonstrate a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and Xenon arc lamp illumination. Our Bayesian analysis of blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores which may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame, and unifies the analysis of localization from blinking and bleaching events. By modeling the entire dataset we are able to use each reappearance of a fluorophore to improve the localization accuracy. The high performance of this technique allows us to reveal the nanoscale dynamics of podosome formation and dissociation with a resolution of 50 nm on a four second timescale. 2011-12-04 /pmc/articles/PMC3272474/ /pubmed/22138825 http://dx.doi.org/10.1038/nmeth.1812 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Cox, Susan Rosten, Edward Monypenny, James Jovanovic-Talisman, Tijana Burnette, Dylan T. Lippincott-Schwartz, Jennifer Jones, Gareth E. Heintzmann, Rainer
spellingShingle	Cox, Susan Rosten, Edward Monypenny, James Jovanovic-Talisman, Tijana Burnette, Dylan T. Lippincott-Schwartz, Jennifer Jones, Gareth E. Heintzmann, Rainer Bayesian localisation microscopy reveals nanoscale podosome dynamics
author_facet	Cox, Susan Rosten, Edward Monypenny, James Jovanovic-Talisman, Tijana Burnette, Dylan T. Lippincott-Schwartz, Jennifer Jones, Gareth E. Heintzmann, Rainer
author_sort	Cox, Susan
title	Bayesian localisation microscopy reveals nanoscale podosome dynamics
title_short	Bayesian localisation microscopy reveals nanoscale podosome dynamics
title_full	Bayesian localisation microscopy reveals nanoscale podosome dynamics
title_fullStr	Bayesian localisation microscopy reveals nanoscale podosome dynamics
title_full_unstemmed	Bayesian localisation microscopy reveals nanoscale podosome dynamics
title_sort	bayesian localisation microscopy reveals nanoscale podosome dynamics
description	We demonstrate a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and Xenon arc lamp illumination. Our Bayesian analysis of blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores which may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame, and unifies the analysis of localization from blinking and bleaching events. By modeling the entire dataset we are able to use each reappearance of a fluorophore to improve the localization accuracy. The high performance of this technique allows us to reveal the nanoscale dynamics of podosome formation and dissociation with a resolution of 50 nm on a four second timescale.
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272474/
_version_	1611503797293547520

Bayesian localisation microscopy reveals nanoscale podosome dynamics

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