Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell c...

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Main Authors:	Jin, Meihua, Zhao, Wennan, Zhang, Yanwen, Kobayashi, Motomasa, Duan, Hongquan, Kong, Dexin
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/

id	pubmed-3233409
recordtype	oai_dc
spelling	pubmed-32334092011-12-15 Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin Article We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells. Molecular Diversity Preservation International (MDPI) 2011-10-26 /pmc/articles/PMC3233409/ /pubmed/22174603 http://dx.doi.org/10.3390/ijms12117352 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin
spellingShingle	Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
author_facet	Jin, Meihua Zhao, Wennan Zhang, Yanwen Kobayashi, Motomasa Duan, Hongquan Kong, Dexin
author_sort	Jin, Meihua
title	Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_short	Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_full	Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_fullStr	Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_full_unstemmed	Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells
title_sort	antiproliferative effect of aaptamine on human chronic myeloid leukemia k562 cells
description	We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233409/
_version_	1611492656795353088

Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

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