Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways

As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adapt...

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Main Authors:	Babu, Mohan, Díaz-Mejía, J. Javier, Vlasblom, James, Gagarinova, Alla, Phanse, Sadhna, Graham, Chris, Yousif, Fouad, Ding, Huiming, Xiong, Xuejian, Nazarians-Armavil, Anaies, Alamgir, Md, Ali, Mehrab, Pogoutse, Oxana, Pe'er, Asaf, Arnold, Roland, Michaut, Magali, Parkinson, John, Golshani, Ashkan, Whitfield, Chris, Wodak, Shoshana J., Moreno-Hagelsieb, Gabriel, Greenblatt, Jack F., Emili, Andrew
Format:	Online
Language:	English
Published:	Public Library of Science 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219608/

id	pubmed-3219608
recordtype	oai_dc
spelling	pubmed-32196082011-11-28 Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways Babu, Mohan Díaz-Mejía, J. Javier Vlasblom, James Gagarinova, Alla Phanse, Sadhna Graham, Chris Yousif, Fouad Ding, Huiming Xiong, Xuejian Nazarians-Armavil, Anaies Alamgir, Md Ali, Mehrab Pogoutse, Oxana Pe'er, Asaf Arnold, Roland Michaut, Magali Parkinson, John Golshani, Ashkan Whitfield, Chris Wodak, Shoshana J. Moreno-Hagelsieb, Gabriel Greenblatt, Jack F. Emili, Andrew Research Article As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among >235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target. Public Library of Science 2011-11-17 /pmc/articles/PMC3219608/ /pubmed/22125496 http://dx.doi.org/10.1371/journal.pgen.1002377 Text en Babu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Babu, Mohan Díaz-Mejía, J. Javier Vlasblom, James Gagarinova, Alla Phanse, Sadhna Graham, Chris Yousif, Fouad Ding, Huiming Xiong, Xuejian Nazarians-Armavil, Anaies Alamgir, Md Ali, Mehrab Pogoutse, Oxana Pe'er, Asaf Arnold, Roland Michaut, Magali Parkinson, John Golshani, Ashkan Whitfield, Chris Wodak, Shoshana J. Moreno-Hagelsieb, Gabriel Greenblatt, Jack F. Emili, Andrew
spellingShingle	Babu, Mohan Díaz-Mejía, J. Javier Vlasblom, James Gagarinova, Alla Phanse, Sadhna Graham, Chris Yousif, Fouad Ding, Huiming Xiong, Xuejian Nazarians-Armavil, Anaies Alamgir, Md Ali, Mehrab Pogoutse, Oxana Pe'er, Asaf Arnold, Roland Michaut, Magali Parkinson, John Golshani, Ashkan Whitfield, Chris Wodak, Shoshana J. Moreno-Hagelsieb, Gabriel Greenblatt, Jack F. Emili, Andrew Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
author_facet	Babu, Mohan Díaz-Mejía, J. Javier Vlasblom, James Gagarinova, Alla Phanse, Sadhna Graham, Chris Yousif, Fouad Ding, Huiming Xiong, Xuejian Nazarians-Armavil, Anaies Alamgir, Md Ali, Mehrab Pogoutse, Oxana Pe'er, Asaf Arnold, Roland Michaut, Magali Parkinson, John Golshani, Ashkan Whitfield, Chris Wodak, Shoshana J. Moreno-Hagelsieb, Gabriel Greenblatt, Jack F. Emili, Andrew
author_sort	Babu, Mohan
title	Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
title_short	Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
title_full	Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
title_fullStr	Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
title_full_unstemmed	Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways
title_sort	genetic interaction maps in escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways
description	As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among >235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.
publisher	Public Library of Science
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219608/
_version_	1611488710950387712

Genetic Interaction Maps in Escherichia coli Reveal Functional Crosstalk among Cell Envelope Biogenesis Pathways

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