Bod1, a novel kinetochore protein required for chromosome biorientation
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein conserved throughout metazoans that associate...
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| Format: | Online |
| Language: | English |
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The Rockefeller University Press
2007
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064755/ |
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pubmed-20647552008-04-22 Bod1, a novel kinetochore protein required for chromosome biorientation Porter, Iain M. McClelland, Sarah E. Khoudoli, Guennadi A. Hunter, Christopher J. Andersen, Jens S. McAinsh, Andrew D. Blow, J. Julian Swedlow, Jason R. Research Articles We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. Bod1-depleted cells form syntelic attachments that can oscillate and generate enough force to separate sister kinetochores, suggesting that microtubule–kinetochore interactions were intact. Releasing Bod1-depleted cells from a monastrol block increases the frequency of syntelic attachments and the number of cells displaying biorientation defects. Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. The Rockefeller University Press 2007-10-22 /pmc/articles/PMC2064755/ /pubmed/17938248 http://dx.doi.org/10.1083/jcb.200704098 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Porter, Iain M. McClelland, Sarah E. Khoudoli, Guennadi A. Hunter, Christopher J. Andersen, Jens S. McAinsh, Andrew D. Blow, J. Julian Swedlow, Jason R. |
| spellingShingle |
Porter, Iain M. McClelland, Sarah E. Khoudoli, Guennadi A. Hunter, Christopher J. Andersen, Jens S. McAinsh, Andrew D. Blow, J. Julian Swedlow, Jason R. Bod1, a novel kinetochore protein required for chromosome biorientation |
| author_facet |
Porter, Iain M. McClelland, Sarah E. Khoudoli, Guennadi A. Hunter, Christopher J. Andersen, Jens S. McAinsh, Andrew D. Blow, J. Julian Swedlow, Jason R. |
| author_sort |
Porter, Iain M. |
| title |
Bod1, a novel kinetochore protein required for chromosome biorientation |
| title_short |
Bod1, a novel kinetochore protein required for chromosome biorientation |
| title_full |
Bod1, a novel kinetochore protein required for chromosome biorientation |
| title_fullStr |
Bod1, a novel kinetochore protein required for chromosome biorientation |
| title_full_unstemmed |
Bod1, a novel kinetochore protein required for chromosome biorientation |
| title_sort |
bod1, a novel kinetochore protein required for chromosome biorientation |
| description |
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. Bod1-depleted cells form syntelic attachments that can oscillate and generate enough force to separate sister kinetochores, suggesting that microtubule–kinetochore interactions were intact. Releasing Bod1-depleted cells from a monastrol block increases the frequency of syntelic attachments and the number of cells displaying biorientation defects. Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. |
| publisher |
The Rockefeller University Press |
| publishDate |
2007 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064755/ |
| _version_ |
1611406821189222400 |