Electrophoretic mobility of Tween 80-encapsulated agarwood oil in aqueous
A study on the feasibility of using gel electrophoresis technique in grading the agarwood oil quality was investigated. Prior to electrophoresis, the emulsified agarwood oil droplets were screened by a diffuse layer of ions that have equal absolute charge to that of the droplets surface charge in aq...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50523/ http://psasir.upm.edu.my/id/eprint/50523/ http://psasir.upm.edu.my/id/eprint/50523/1/%2820%29%20IFRJ-16433%20Naim.pdf |
| Summary: | A study on the feasibility of using gel electrophoresis technique in grading the agarwood oil quality was investigated. Prior to electrophoresis, the emulsified agarwood oil droplets were screened by a diffuse layer of ions that have equal absolute charge to that of the droplets surface charge in aqueous phase. The condition was obtained by varying the concentrations of non-ionic surfactant; Tween 80 until the critical aggregation concentration (CAC) value of 0.0167% (v/v) was achieved. The prepared droplets suspended in the aqueous within nano-metre size and had ability to migrate through the agarose gel with its own specific electrophoretic mobility. However, due to the limitation of gel pore size, only large oil droplets (>200 nm) indicated visible bands. Overall, a novel work for grading the emulsified agarwood oil droplet with its own electrical properties was feasible. |
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