Lactic Acid production using alkaliphile lactic acid bacterium
Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and presence of contamina...
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| Format: | Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, UNIMAS
2012
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| Online Access: | http://ir.unimas.my/6206/ http://ir.unimas.my/6206/1/LACTIC%20ACID%20PRODUCTION%20USING%20ALKALIPHILE%20LACTIC%20ACID%20BACTERIUM%2824pgs%29.pdf http://ir.unimas.my/6206/7/LACTIC%20ACID%20PRODUCTION%20USING%20ALKALIPHILE%20LACTIC%20ACID%20BACTERIUM%28OCR%29.pdf |
| Summary: | Batch fermentation of glucose to lactic acid was conducted using new isolated bacteria from
laboratory of Biofuel R&D (UNIMAS) at alkali pH of 9 and 10. Commercial glucose was
used to produce lactic acid and the effect of alkali pH on biomass, lactic acid production, and
presence of contamination were investigated. Batch fermentation system was done at 37 °C
and agitated at 250 rpm with controlled pH environment. Fermentation at pH 9 gave high
production biomass and lactic acid of about 1.80 g/L and 45.21 g/L while less production is
shown at pH 10 with 1.27 g/L and 42.66 g/L. Spread plate of both fermentation show growth
only single colony which no contamination. Consequently, it shows that high pH affect cell
growth, lactic acid production and prevents contamination. Complete fermentation at pH 9
show is more favourable with high rate production of lactic acid in 42 h compared to at pH 10
in 72 h. |
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