Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002

The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature,...

Full description

Bibliographic Details
Main Authors:	Anwarali, F. A., Awang Husaini, Awang Ahmad Sallehin
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2006
Subjects:	AC Collections. Series. Collected works
Online Access:	http://ir.unimas.my/2149/ http://ir.unimas.my/2149/ http://ir.unimas.my/2149/1/Enhancing%20a%20-Amylase%20and%20Cellulase%20in%20vivo%20enzyme%20expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacillus%20amyloliquefaciens%20UMAS%201002.pdf

id	unimas-2149
recordtype	eprints
spelling	unimas-21492017-03-06T07:21:51Z http://ir.unimas.my/2149/ Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002 Anwarali, F. A. Awang Husaini, Awang Ahmad Sallehin AC Collections. Series. Collected works The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of α-amylase and cellulase was at 14.19 and 13.15 IU mL-1, respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for α-amylase and cellulase, respectively. Enzyme activity was 2.8 (10.80 IU mL-1) and 3.2 (9.38 IU mL-1) fold higher for α-amylase and cellulase respectively when 1% w/v soluble starch was applied as additive substrates with 0.5% hampas. However in optimal media that consist of 4%w/v of hampas, addition of 1% w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of α-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, Dissolved Oxygen (DO) and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too. Asian Network for Scientific Information 2006 Article NonPeerReviewed text en http://ir.unimas.my/2149/1/Enhancing%20a%20-Amylase%20and%20Cellulase%20in%20vivo%20enzyme%20expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacillus%20amyloliquefaciens%20UMAS%201002.pdf Anwarali, F. A. and Awang Husaini, Awang Ahmad Sallehin (2006) Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002. Biotechnology 5(3) : 391-403, 2006, 5 (3). pp. 391-403. ISSN 1682-296x DOI: 10.3923/biotech.2006.391.403
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Sarawak
building	UNIMAS Institutional Repository
collection	Online Access
language	English
topic	AC Collections. Series. Collected works
spellingShingle	AC Collections. Series. Collected works Anwarali, F. A. Awang Husaini, Awang Ahmad Sallehin Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
description	The effect of Solid State Fermentation (SSF) parameters on the production of extracellular α-amylase and cellulase (endoglucanases) by Bacillus amyloliquefaciens UMAS 1002 using sago pith residue hampas in shaker flasks was investigated in this study. The appropriate incubation period, temperature, pH, agitation speed, inoculum concentration, hampas concentration and additive substrate effect were optimized for enzyme production. The activity of α-amylase and cellulase was at 14.19 and 13.15 IU mL-1, respectively in optimal culture medium. Maximum yield for both enzymes were achieved by employing 4% w/v hampas in 0.2 M citrate buffer at pH 6 and incubated at 40°C for 6 h with agitation speed of 100 rpm. Inoculum concentrations were found to be optimum at 3% v/v and 4% v/v for α-amylase and cellulase, respectively. Enzyme activity was 2.8 (10.80 IU mL-1) and 3.2 (9.38 IU mL-1) fold higher for α-amylase and cellulase respectively when 1% w/v soluble starch was applied as additive substrates with 0.5% hampas. However in optimal media that consist of 4%w/v of hampas, addition of 1% w/v soluble starch intend to inhibit both enzyme productions. Result revealed that temperature, pH and shaking condition were the most significant factors for the production of α-amylase and cellulase enzyme. Temperature influenced enzyme production by affecting the other parameters including bacterial growth, pH, Dissolved Oxygen (DO) and reducing sugars. Nevertheless, shaking condition could affect DO concentration that in turn affected bacterial growth and enzymes production too.
format	Article
author	Anwarali, F. A. Awang Husaini, Awang Ahmad Sallehin
author_facet	Anwarali, F. A. Awang Husaini, Awang Ahmad Sallehin
author_sort	Anwarali, F. A.
title	Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
title_short	Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
title_full	Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
title_fullStr	Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
title_full_unstemmed	Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002
title_sort	enhancing a -amylase and cellulase in vivo enzyme expressions on sago pith residue using bacillus amyloliquefaciens umas 1002
publisher	Asian Network for Scientific Information
publishDate	2006
url	http://ir.unimas.my/2149/ http://ir.unimas.my/2149/ http://ir.unimas.my/2149/1/Enhancing%20a%20-Amylase%20and%20Cellulase%20in%20vivo%20enzyme%20expressions%20on%20Sago%20Pith%20Residue%20Using%20Bacillus%20amyloliquefaciens%20UMAS%201002.pdf
first_indexed	2018-09-06T14:48:50Z
last_indexed	2018-09-06T14:48:50Z
_version_	1610869983478284288

Enhancing a -Amylase and Cellulase in vivo enzyme expressions on Sago Pith Residue Using Bacillus amyloliquefaciens UMAS 1002

Similar Items