The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus

Telomerase replicates chromosome ends, a function necessary for maintaining genome integrity. We have identified the gene that encodes the catalytic reverse transcriptase (RT) component of this enzyme in the malaria parasite Plasmodium falciparum (PfTERT) as well as the orthologous genes from two ro...

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Main Authors:	Figueiredo, Luisa M., Rocha, Eduardo P. C., Mancio-Silva, Liliana, Prevost, Christine, Hernandez-Verdun, Danièle, Scherf, Artur
Format:	Online
Language:	English
Published:	Oxford University Press 2005
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549419/

id	pubmed-549419
recordtype	oai_dc
spelling	pubmed-5494192005-02-24 The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus Figueiredo, Luisa M. Rocha, Eduardo P. C. Mancio-Silva, Liliana Prevost, Christine Hernandez-Verdun, Danièle Scherf, Artur Article Telomerase replicates chromosome ends, a function necessary for maintaining genome integrity. We have identified the gene that encodes the catalytic reverse transcriptase (RT) component of this enzyme in the malaria parasite Plasmodium falciparum (PfTERT) as well as the orthologous genes from two rodent and one simian malaria species. PfTERT is predicted to encode a basic protein that contains the major sequence motifs previously identified in known telomerase RTs (TERTs). At ∼2500 amino acids, PfTERT is three times larger than other characterized TERTs. We observed remarkable sequence diversity between TERT proteins of different Plasmodial species, with conserved domains alternating with hypervariable regions. Immunofluorescence analysis revealed that PfTERT is expressed in asexual blood stage parasites that have begun DNA synthesis. Surprisingly, rather than at telomere clusters, PfTERT typically localizes into a discrete nuclear compartment. We further demonstrate that this compartment is associated with the nucleolus, hereby defined for the first time in P.falciparum. Oxford University Press 2005 2005-02-18 /pmc/articles/PMC549419/ /pubmed/15722485 http://dx.doi.org/10.1093/nar/gki260 Text en © The Author 2005. Published by Oxford University Press. All rights reserved
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Figueiredo, Luisa M. Rocha, Eduardo P. C. Mancio-Silva, Liliana Prevost, Christine Hernandez-Verdun, Danièle Scherf, Artur
spellingShingle	Figueiredo, Luisa M. Rocha, Eduardo P. C. Mancio-Silva, Liliana Prevost, Christine Hernandez-Verdun, Danièle Scherf, Artur The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
author_facet	Figueiredo, Luisa M. Rocha, Eduardo P. C. Mancio-Silva, Liliana Prevost, Christine Hernandez-Verdun, Danièle Scherf, Artur
author_sort	Figueiredo, Luisa M.
title	The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
title_short	The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
title_full	The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
title_fullStr	The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
title_full_unstemmed	The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
title_sort	unusually large plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus
description	Telomerase replicates chromosome ends, a function necessary for maintaining genome integrity. We have identified the gene that encodes the catalytic reverse transcriptase (RT) component of this enzyme in the malaria parasite Plasmodium falciparum (PfTERT) as well as the orthologous genes from two rodent and one simian malaria species. PfTERT is predicted to encode a basic protein that contains the major sequence motifs previously identified in known telomerase RTs (TERTs). At ∼2500 amino acids, PfTERT is three times larger than other characterized TERTs. We observed remarkable sequence diversity between TERT proteins of different Plasmodial species, with conserved domains alternating with hypervariable regions. Immunofluorescence analysis revealed that PfTERT is expressed in asexual blood stage parasites that have begun DNA synthesis. Surprisingly, rather than at telomere clusters, PfTERT typically localizes into a discrete nuclear compartment. We further demonstrate that this compartment is associated with the nucleolus, hereby defined for the first time in P.falciparum.
publisher	Oxford University Press
publishDate	2005
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549419/
_version_	1611370980274339840

The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus

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