Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control

The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS...

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Main Authors: Glytsou, Christina, Calvo, Enrique, Cogliati, Sara, Mehrotra, Arpit, Anastasia, Irene, Rigoni, Giovanni, Raimondi, Andrea, Shintani, Norihito, Loureiro, Marta, Vazquez, Jesùs, Pellegrini, Luca, Enriquez, Jose Antonio, Scorrano, Luca, Soriano, Maria Eugenia
Format: Online
Language:English
Published: Cell Press 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/
id pubmed-5186903
recordtype oai_dc
spelling pubmed-51869032017-01-03 Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control Glytsou, Christina Calvo, Enrique Cogliati, Sara Mehrotra, Arpit Anastasia, Irene Rigoni, Giovanni Raimondi, Andrea Shintani, Norihito Loureiro, Marta Vazquez, Jesùs Pellegrini, Luca Enriquez, Jose Antonio Scorrano, Luca Soriano, Maria Eugenia Article The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS. Cell Press 2016-12-13 /pmc/articles/PMC5186903/ /pubmed/27974214 http://dx.doi.org/10.1016/j.celrep.2016.11.049 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
spellingShingle Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
author_facet Glytsou, Christina
Calvo, Enrique
Cogliati, Sara
Mehrotra, Arpit
Anastasia, Irene
Rigoni, Giovanni
Raimondi, Andrea
Shintani, Norihito
Loureiro, Marta
Vazquez, Jesùs
Pellegrini, Luca
Enriquez, Jose Antonio
Scorrano, Luca
Soriano, Maria Eugenia
author_sort Glytsou, Christina
title Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_short Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_full Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_fullStr Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_full_unstemmed Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control
title_sort optic atrophy 1 is epistatic to the core micos component mic60 in mitochondrial cristae shape control
description The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.
publisher Cell Press
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5186903/
_version_ 1613824946231836672

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