Labeling proteins inside living cells using external fluorophores for microscopy
Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial toxin which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG’s to small ligands, with high effi...
| Main Authors: | , , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
eLife Sciences Publications, Ltd
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148600/ |