Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy

Fluorophore excited state lifetime is a useful indicator of micro-environment in cellular optical molecular imaging. For quantitative sensing, precise lifetime determination is important, yet is often difficult to accomplish when using the experimental conditions favored by live cells. Here we repor...

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Bibliographic Details
Main Authors: Chang, Ching-Wei, Mycek, Mary-Ann
Format: Online
Language:English
Published: Optical Society of America 2010
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3410727/