Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation
While transformation of normal cells to cancer cells is accompanied with a switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis, it is interesting to ask if cancer cells can revert from Warburg effect to OXPHOS. Our previous works suggested that cancer cells reverted to OXPHOS, when...
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| Format: | Online |
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Impact Journals LLC
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130031/ |
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pubmed-51300312016-12-11 Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation Wu, Hao Ying, Minfeng Hu, Xun Research Paper While transformation of normal cells to cancer cells is accompanied with a switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis, it is interesting to ask if cancer cells can revert from Warburg effect to OXPHOS. Our previous works suggested that cancer cells reverted to OXPHOS, when they were exposed to lactic acidosis, a common factor in tumor environment. However, the conclusion cannot be drawn unless ATP output from glycolysis and OXPHOS is quantitatively determined. Here we quantitatively measured ATP generation from glycolysis and OXPHOS in 9 randomly selected cancer cell lines. Without lactic acidosis, glycolysis and OXPHOS generated 23.7% − 52.2 % and 47.8% − 76.3% of total ATP, respectively; with lactic acidosis (20 mM lactate with pH 6.7), glycolysis and OXPHOS provided 5.7% − 13.4% and 86.6% − 94.3% of total ATP. We concluded that cancer cells under lactic acidosis reverted from Warburg effect to OXPHOS phenotype. Impact Journals LLC 2016-05-31 /pmc/articles/PMC5130031/ /pubmed/27259254 http://dx.doi.org/10.18632/oncotarget.9746 Text en Copyright: © 2016 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wu, Hao Ying, Minfeng Hu, Xun |
| spellingShingle |
Wu, Hao Ying, Minfeng Hu, Xun Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| author_facet |
Wu, Hao Ying, Minfeng Hu, Xun |
| author_sort |
Wu, Hao |
| title |
Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| title_short |
Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| title_full |
Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| title_fullStr |
Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| title_full_unstemmed |
Lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| title_sort |
lactic acidosis switches cancer cells from aerobic glycolysis back to dominant oxidative phosphorylation |
| description |
While transformation of normal cells to cancer cells is accompanied with a switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis, it is interesting to ask if cancer cells can revert from Warburg effect to OXPHOS. Our previous works suggested that cancer cells reverted to OXPHOS, when they were exposed to lactic acidosis, a common factor in tumor environment. However, the conclusion cannot be drawn unless ATP output from glycolysis and OXPHOS is quantitatively determined. Here we quantitatively measured ATP generation from glycolysis and OXPHOS in 9 randomly selected cancer cell lines. Without lactic acidosis, glycolysis and OXPHOS generated 23.7% − 52.2 % and 47.8% − 76.3% of total ATP, respectively; with lactic acidosis (20 mM lactate with pH 6.7), glycolysis and OXPHOS provided 5.7% − 13.4% and 86.6% − 94.3% of total ATP. We concluded that cancer cells under lactic acidosis reverted from Warburg effect to OXPHOS phenotype. |
| publisher |
Impact Journals LLC |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130031/ |
| _version_ |
1613748312647663616 |