Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening...

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Main Authors:	Zhao, Chong, Sakaguchi, Takenori, Fujita, Kosuke, Ito, Hideyuki, Nishida, Norihisa, Nagatomo, Akifumi, Tanaka-Azuma, Yukimasa, Katakura, Yoshinori
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093269/

id	pubmed-5093269
recordtype	oai_dc
spelling	pubmed-50932692016-11-13 Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation Zhao, Chong Sakaguchi, Takenori Fujita, Kosuke Ito, Hideyuki Nishida, Norihisa Nagatomo, Akifumi Tanaka-Azuma, Yukimasa Katakura, Yoshinori Research Article Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A. Hindawi Publishing Corporation 2016 2016-10-20 /pmc/articles/PMC5093269/ /pubmed/27840668 http://dx.doi.org/10.1155/2016/2927131 Text en Copyright © 2016 Chong Zhao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhao, Chong Sakaguchi, Takenori Fujita, Kosuke Ito, Hideyuki Nishida, Norihisa Nagatomo, Akifumi Tanaka-Azuma, Yukimasa Katakura, Yoshinori
spellingShingle	Zhao, Chong Sakaguchi, Takenori Fujita, Kosuke Ito, Hideyuki Nishida, Norihisa Nagatomo, Akifumi Tanaka-Azuma, Yukimasa Katakura, Yoshinori Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
author_facet	Zhao, Chong Sakaguchi, Takenori Fujita, Kosuke Ito, Hideyuki Nishida, Norihisa Nagatomo, Akifumi Tanaka-Azuma, Yukimasa Katakura, Yoshinori
author_sort	Zhao, Chong
title	Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
title_short	Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
title_full	Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
title_fullStr	Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
title_full_unstemmed	Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation
title_sort	pomegranate-derived polyphenols reduce reactive oxygen species production via sirt3-mediated sod2 activation
description	Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A.
publisher	Hindawi Publishing Corporation
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093269/
_version_	1613710647896309760

Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

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