Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

The canonical set of amino acids leads to an exceptionally wide range of protein functionality. Nevertheless, the set of residues still imposes limitations on potential protein applications. The incorporation of noncanonical amino acids can enlarge this scope. There are two complementary approaches...

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Bibliographic Details
Main Authors: Worst, Emanuel G., Exner, Matthias P., De Simone, Alessandro, Schenkelberger, Marc, Noireaux, Vincent, Budisa, Nediljko, Ott, Albrecht
Format: Online
Language:English
Published: MyJove Corporation 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091720/