The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis

The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis

Anti-endothelial cell antibodies (AECA) are frequently detected in patients with systemic lupus erythematosus (SLE), but their pathological role remains unclear. We recently developed a solubilized cell surface protein capture enzyme-linked immunosorbent assay (CSP-ELISA) to detect antibodies agains...

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Main Authors: Kondo, Ayako, Takahashi, Kazuo, Mizuno, Tomohiro, Kato, Akihiro, Hirano, Daisuke, Yamamoto, Naoki, Hayashi, Hiroki, Koide, Shigehisa, Takahashi, Hiroshi, Hasegawa, Midori, Hiki, Yoshiyuki, Yoshida, Shunji, Miura, Keiji, Yuzawa, Yukio
Format: Online
Language:English
Published: Public Library of Science 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082850/
id pubmed-5082850
recordtype oai_dc
spelling pubmed-50828502016-11-04 The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis Kondo, Ayako Takahashi, Kazuo Mizuno, Tomohiro Kato, Akihiro Hirano, Daisuke Yamamoto, Naoki Hayashi, Hiroki Koide, Shigehisa Takahashi, Hiroshi Hasegawa, Midori Hiki, Yoshiyuki Yoshida, Shunji Miura, Keiji Yuzawa, Yukio Research Article Anti-endothelial cell antibodies (AECA) are frequently detected in patients with systemic lupus erythematosus (SLE), but their pathological role remains unclear. We recently developed a solubilized cell surface protein capture enzyme-linked immunosorbent assay (CSP-ELISA) to detect antibodies against membrane proteins involved in autoimmune reactions. In this study, sera from 51 patients with biopsy-proven lupus nephritis (LN), 25 with SLE without renal involvement (non-LN SLE), 42 disease control (DC) subjects, and 80 healthy control (HC) subjects were tested for IgG- and IgA-AECA for human umbilical vein endothelial cells (HUVEC) and human glomerular EC (HGEC) by using CSP-ELISA. IgG- and IgA-AECA titers were significantly higher in LN and non-LN SLE patients than in the DC or HC (P < 0.001) groups. IgG- and IgA-AECA titers for HUVEC corresponded well with those for HGEC. The IgA-AECA level correlated with the SLE disease activity index and with histological evidence of active lesions (cellular proliferations, hyaline thrombi and wire loops, leukocytic infiltration, and fibrinoid necrosis) in LN patients (P < 0.001). The sensitivity of IgA-AECA as a diagnostic test for histological evidence of active lesions in LN patients was 0.92, with a specificity of 0.70. The significant correlation of IgA-AECA with glomerular hypercellularity indicates that IgA-AECA are associated with endothelial damage in LN. Public Library of Science 2016-10-27 /pmc/articles/PMC5082850/ /pubmed/27788140 http://dx.doi.org/10.1371/journal.pone.0163085 Text en © 2016 Kondo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Kondo, Ayako
Takahashi, Kazuo
Mizuno, Tomohiro
Kato, Akihiro
Hirano, Daisuke
Yamamoto, Naoki
Hayashi, Hiroki
Koide, Shigehisa
Takahashi, Hiroshi
Hasegawa, Midori
Hiki, Yoshiyuki
Yoshida, Shunji
Miura, Keiji
Yuzawa, Yukio
spellingShingle Kondo, Ayako
Takahashi, Kazuo
Mizuno, Tomohiro
Kato, Akihiro
Hirano, Daisuke
Yamamoto, Naoki
Hayashi, Hiroki
Koide, Shigehisa
Takahashi, Hiroshi
Hasegawa, Midori
Hiki, Yoshiyuki
Yoshida, Shunji
Miura, Keiji
Yuzawa, Yukio
The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
author_facet Kondo, Ayako
Takahashi, Kazuo
Mizuno, Tomohiro
Kato, Akihiro
Hirano, Daisuke
Yamamoto, Naoki
Hayashi, Hiroki
Koide, Shigehisa
Takahashi, Hiroshi
Hasegawa, Midori
Hiki, Yoshiyuki
Yoshida, Shunji
Miura, Keiji
Yuzawa, Yukio
author_sort Kondo, Ayako
title The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
title_short The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
title_full The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
title_fullStr The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
title_full_unstemmed The Level of IgA Antibodies to Endothelial Cells Correlates with Histological Evidence of Disease Activity in Patients with Lupus Nephritis
title_sort level of iga antibodies to endothelial cells correlates with histological evidence of disease activity in patients with lupus nephritis
description Anti-endothelial cell antibodies (AECA) are frequently detected in patients with systemic lupus erythematosus (SLE), but their pathological role remains unclear. We recently developed a solubilized cell surface protein capture enzyme-linked immunosorbent assay (CSP-ELISA) to detect antibodies against membrane proteins involved in autoimmune reactions. In this study, sera from 51 patients with biopsy-proven lupus nephritis (LN), 25 with SLE without renal involvement (non-LN SLE), 42 disease control (DC) subjects, and 80 healthy control (HC) subjects were tested for IgG- and IgA-AECA for human umbilical vein endothelial cells (HUVEC) and human glomerular EC (HGEC) by using CSP-ELISA. IgG- and IgA-AECA titers were significantly higher in LN and non-LN SLE patients than in the DC or HC (P < 0.001) groups. IgG- and IgA-AECA titers for HUVEC corresponded well with those for HGEC. The IgA-AECA level correlated with the SLE disease activity index and with histological evidence of active lesions (cellular proliferations, hyaline thrombi and wire loops, leukocytic infiltration, and fibrinoid necrosis) in LN patients (P < 0.001). The sensitivity of IgA-AECA as a diagnostic test for histological evidence of active lesions in LN patients was 0.92, with a specificity of 0.70. The significant correlation of IgA-AECA with glomerular hypercellularity indicates that IgA-AECA are associated with endothelial damage in LN.
publisher Public Library of Science
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5082850/
_version_ 1613700726739959808

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