Unusual Salt and pH Induced Changes in Polyethylenimine Solutions

Linear PEI is a cationic polymer commonly used for complexing DNA into nanoparticles for cell-transfection and gene-therapy applications. The polymer has closely-spaced amines with weak-base protonation capacity, and a hydrophobic backbone that is kept unaggregated by intra-chain repulsion. As a res...

Full description

Bibliographic Details
Main Authors:	Curtis, Kimberly A., Miller, Danielle, Millard, Paul, Basu, Saswati, Horkay, Ferenc, Chandran, Preethi L
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042459/

id	pubmed-5042459
recordtype	oai_dc
spelling	pubmed-50424592016-10-27 Unusual Salt and pH Induced Changes in Polyethylenimine Solutions Curtis, Kimberly A. Miller, Danielle Millard, Paul Basu, Saswati Horkay, Ferenc Chandran, Preethi L Research Article Linear PEI is a cationic polymer commonly used for complexing DNA into nanoparticles for cell-transfection and gene-therapy applications. The polymer has closely-spaced amines with weak-base protonation capacity, and a hydrophobic backbone that is kept unaggregated by intra-chain repulsion. As a result, in solution PEI exhibits multiple buffering mechanisms, and polyelectrolyte states that shift between aggregated and free forms. We studied the interplay between the aggregation and protonation behavior of 2.5 kDa linear PEI by pH probing, vapor pressure osmometry, dynamic light scattering, and ninhydrin assay. Our results indicate that: Public Library of Science 2016-09-29 /pmc/articles/PMC5042459/ /pubmed/27685846 http://dx.doi.org/10.1371/journal.pone.0158147 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Curtis, Kimberly A. Miller, Danielle Millard, Paul Basu, Saswati Horkay, Ferenc Chandran, Preethi L
spellingShingle	Curtis, Kimberly A. Miller, Danielle Millard, Paul Basu, Saswati Horkay, Ferenc Chandran, Preethi L Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
author_facet	Curtis, Kimberly A. Miller, Danielle Millard, Paul Basu, Saswati Horkay, Ferenc Chandran, Preethi L
author_sort	Curtis, Kimberly A.
title	Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
title_short	Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
title_full	Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
title_fullStr	Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
title_full_unstemmed	Unusual Salt and pH Induced Changes in Polyethylenimine Solutions
title_sort	unusual salt and ph induced changes in polyethylenimine solutions
description	Linear PEI is a cationic polymer commonly used for complexing DNA into nanoparticles for cell-transfection and gene-therapy applications. The polymer has closely-spaced amines with weak-base protonation capacity, and a hydrophobic backbone that is kept unaggregated by intra-chain repulsion. As a result, in solution PEI exhibits multiple buffering mechanisms, and polyelectrolyte states that shift between aggregated and free forms. We studied the interplay between the aggregation and protonation behavior of 2.5 kDa linear PEI by pH probing, vapor pressure osmometry, dynamic light scattering, and ninhydrin assay. Our results indicate that:
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042459/
_version_	1613666014468243456

Unusual Salt and pH Induced Changes in Polyethylenimine Solutions

Similar Items