Flexible and rapid construction of viral chimeras applied to hepatitis C virus
A novel and broadly applicable strategy combining site-directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using hepatitis C virus (HCV) as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites...
| Main Authors: | , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Microbiology Society
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042125/ |