Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus
A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites, were...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Microbiology Society
2016
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| Online Access: | http://eprints.nottingham.ac.uk/34493/ http://eprints.nottingham.ac.uk/34493/ http://eprints.nottingham.ac.uk/34493/ http://eprints.nottingham.ac.uk/34493/1/jgv%200%20000530.pdf http://eprints.nottingham.ac.uk/34493/8/2187_jgv000530.%20Flexible.pdf |