A quick guide to CRISPR sgRNA design tools

A quick guide to CRISPR sgRNA design tools

Targeted genome editing is now possible in nearly any organism and is widely acknowledged as a biotech game-changer. Among available gene editing techniques, the CRISPR-Cas9 system is the current favorite because it has been shown to work in many species, does not necessarily result in the addition...

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Main Authors: Brazelton, Vincent A, Zarecor, Scott, Wright, David A, Wang, Yuan, Liu, Jie, Chen, Keting, Yang, Bing, Lawrence-Dill, Carolyn J
Format: Online
Language:English
Published: Taylor & Francis 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033207/
id pubmed-5033207
recordtype oai_dc
spelling pubmed-50332072017-01-08 A quick guide to CRISPR sgRNA design tools Brazelton, Vincent A Zarecor, Scott Wright, David A Wang, Yuan Liu, Jie Chen, Keting Yang, Bing Lawrence-Dill, Carolyn J Research Papers Targeted genome editing is now possible in nearly any organism and is widely acknowledged as a biotech game-changer. Among available gene editing techniques, the CRISPR-Cas9 system is the current favorite because it has been shown to work in many species, does not necessarily result in the addition of foreign DNA at the target site, and follows a set of simple design rules for target selection. Use of the CRISPR-Cas9 system is facilitated by the availability of an array of CRISPR design tools that vary in design specifications and parameter choices, available genomes, graphical visualization, and downstream analysis functionality. To help researchers choose a tool that best suits their specific research needs, we review the functionality of various CRISPR design tools including our own, the CRISPR Genome Analysis Tool (CGAT; http://cropbioengineering.iastate.edu/cgat). Taylor & Francis 2016-01-08 /pmc/articles/PMC5033207/ /pubmed/26745836 http://dx.doi.org/10.1080/21645698.2015.1137690 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Brazelton, Vincent A
Zarecor, Scott
Wright, David A
Wang, Yuan
Liu, Jie
Chen, Keting
Yang, Bing
Lawrence-Dill, Carolyn J
spellingShingle Brazelton, Vincent A
Zarecor, Scott
Wright, David A
Wang, Yuan
Liu, Jie
Chen, Keting
Yang, Bing
Lawrence-Dill, Carolyn J
A quick guide to CRISPR sgRNA design tools
author_facet Brazelton, Vincent A
Zarecor, Scott
Wright, David A
Wang, Yuan
Liu, Jie
Chen, Keting
Yang, Bing
Lawrence-Dill, Carolyn J
author_sort Brazelton, Vincent A
title A quick guide to CRISPR sgRNA design tools
title_short A quick guide to CRISPR sgRNA design tools
title_full A quick guide to CRISPR sgRNA design tools
title_fullStr A quick guide to CRISPR sgRNA design tools
title_full_unstemmed A quick guide to CRISPR sgRNA design tools
title_sort quick guide to crispr sgrna design tools
description Targeted genome editing is now possible in nearly any organism and is widely acknowledged as a biotech game-changer. Among available gene editing techniques, the CRISPR-Cas9 system is the current favorite because it has been shown to work in many species, does not necessarily result in the addition of foreign DNA at the target site, and follows a set of simple design rules for target selection. Use of the CRISPR-Cas9 system is facilitated by the availability of an array of CRISPR design tools that vary in design specifications and parameter choices, available genomes, graphical visualization, and downstream analysis functionality. To help researchers choose a tool that best suits their specific research needs, we review the functionality of various CRISPR design tools including our own, the CRISPR Genome Analysis Tool (CGAT; http://cropbioengineering.iastate.edu/cgat).
publisher Taylor & Francis
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5033207/
_version_ 1613658649100550144

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