Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR

Renal interstitial fibrosis is a common pathological process in the progression of kidney disease. A nuclear magnetic resonance (NMR) based metabolomic approach was used to analyze the kidney tissues of rats with renal interstitial fibrosis (RIF), induced by unilateral ureteral obstruction (UUO). Th...

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Main Authors:	Li, Zhenyu, Li, Aiping, Gao, Jining, Li, Hong, Qin, Xuemei
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023943/

id	pubmed-5023943
recordtype	oai_dc
spelling	pubmed-50239432016-09-30 Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR Li, Zhenyu Li, Aiping Gao, Jining Li, Hong Qin, Xuemei Pharmacology Renal interstitial fibrosis is a common pathological process in the progression of kidney disease. A nuclear magnetic resonance (NMR) based metabolomic approach was used to analyze the kidney tissues of rats with renal interstitial fibrosis (RIF), induced by unilateral ureteral obstruction (UUO). The combination of a variety of statistical methods were used to screen out 14 significantly changed potential metabolites, which are related with multiple biochemical processes including amino acid metabolism, adenine metabolism, energy metabolism, osmolyte change and induced oxidative stress. The exploration of the contralateral kidneys enhanced the understanding of the disease, which was also supported by serum biochemistry and kidney histopathology results. In addition, the pathological parameters (clinical chemistry, histological and immunohistochemistry results) were correlated with the significantly changed differential metabolites related with RIF. This study showed that targeted tissue metabolomic analysis can be used as a useful tool to understand the mechanism of the disease and provide a novel insight in the pathogenesis of RIF. Frontiers Media S.A. 2016-09-15 /pmc/articles/PMC5023943/ /pubmed/27695416 http://dx.doi.org/10.3389/fphar.2016.00307 Text en Copyright © 2016 Li, Li, Gao, Li and Qin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Li, Zhenyu Li, Aiping Gao, Jining Li, Hong Qin, Xuemei
spellingShingle	Li, Zhenyu Li, Aiping Gao, Jining Li, Hong Qin, Xuemei Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
author_facet	Li, Zhenyu Li, Aiping Gao, Jining Li, Hong Qin, Xuemei
author_sort	Li, Zhenyu
title	Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
title_short	Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
title_full	Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
title_fullStr	Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
title_full_unstemmed	Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR
title_sort	kidney tissue targeted metabolic profiling of unilateral ureteral obstruction rats by nmr
description	Renal interstitial fibrosis is a common pathological process in the progression of kidney disease. A nuclear magnetic resonance (NMR) based metabolomic approach was used to analyze the kidney tissues of rats with renal interstitial fibrosis (RIF), induced by unilateral ureteral obstruction (UUO). The combination of a variety of statistical methods were used to screen out 14 significantly changed potential metabolites, which are related with multiple biochemical processes including amino acid metabolism, adenine metabolism, energy metabolism, osmolyte change and induced oxidative stress. The exploration of the contralateral kidneys enhanced the understanding of the disease, which was also supported by serum biochemistry and kidney histopathology results. In addition, the pathological parameters (clinical chemistry, histological and immunohistochemistry results) were correlated with the significantly changed differential metabolites related with RIF. This study showed that targeted tissue metabolomic analysis can be used as a useful tool to understand the mechanism of the disease and provide a novel insight in the pathogenesis of RIF.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023943/
_version_	1613652098901082112

Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR

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