Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

Editing plant genomes is technically challenging in hard-to-transform plants and usually involves transgenic intermediates, which causes regulatory concerns. Here we report two simple and efficient genome-editing methods in which plants are regenerated from callus cells transiently expressing CRISPR...

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Bibliographic Details
Main Authors: Zhang, Yi, Liang, Zhen, Zong, Yuan, Wang, Yanpeng, Liu, Jinxing, Chen, Kunling, Qiu, Jin-Long, Gao, Caixia
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007326/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007326/

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